SIPA1L1 Polyklonaler Antikörper

SIPA1L1 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 25086-1-AP

Synonyme

SIPA1 like protein 1, Signal-induced proliferation-associated 1-like protein 1, High-risk human papilloma viruses E6 oncoproteins targeted protein 1, E6TP1, E6-targeted protein 1

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  WB analysis using 25086-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25086-1-AP (SIPA1L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 25086-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25086-1-AP (SIPA1L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 25086-1-AP

  IP result of anti-SIPA1L1 (IP:25086-1-AP, 4ug; Detection:25086-1-AP 1:3000) with mouse brain tissue lysate 1280 ug.

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  IHC staining of human brain using 25086-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25086-1-AP (SIPA1L1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 25086-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25086-1-AP (SIPA1L1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 25086-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 25086-1-AP (SIPA1L1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas using 25086-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 25086-1-AP (SIPA1L1 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HeLa-Zellen, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

25086-1-AP bindet in WB, IHC, IF, IP, ELISA SIPA1L1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SIPA1L1 fusion protein Ag18900
• Vollständiger Name: signal-induced proliferation-associated 1 like 1
• Berechnetes Molekulargewicht: 1803 aa, 200 kDa
• Beobachtetes Molekulargewicht: 200 kDa
• GenBank-Zugangsnummer: BC146777
• Gene symbol: SIPA1L1
• Gene ID (NCBI): 26037
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB,IP	Neuron; Prosapip1-Dependent Synaptic Adaptations in the Nucleus Accumbens Drive Alcohol Intake, Seeking, and Reward.; Authors - Sophie Laguesse
mouse	WB	bioRxiv; Prosapip1 in the dorsal hippocampus mediates synaptic protein composition, long-term potentiation, and spatial memory; Authors - Zachary W Hoisington
human	WB,IF	JCI Insight; Effects of SIPA1L1 on trabecular meshwork extracellular matrix protein accumulation and cellular phagocytosis in POAG; Authors - Chenyu Xu ; KD Validated
mouse	IHC	Synapse; Ginkgo biloba Extract Improves Dendritic Spine Injury in Cerebellar Purkinje Cells Induced by MPTP in Mice by Regulating the PLK2-SPAR Pathway; Authors - Yilin Lyu