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SKIV2L Polyklonaler Antikörper
SKIV2L Polyklonal Antikörper für WB, IHC, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human
Anwendung
WB, IHC, IF, IP, ChIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 11462-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | HEK-293-Zellen, COLO 320-Zellen, HepG2-Zellen, humanes Nierengewebe, Jurkat-Zellen |
| Erfolgreiche IP | HepG2-Zellen |
| Erfolgreiche Detektion in IHC | humanes Magengewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunhistochemie (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 5 publications below |
| WB | See 14 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
| ChIP | See 1 publications below |
Produktinformation
11462-1-AP bindet in WB, IHC, IF, IP, ChIP, ELISA SKIV2L und zeigt Reaktivität mit human
| Getestete Reaktivität | human |
| In Publikationen genannte Reaktivität | human |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | SKIV2L fusion protein Ag1977 |
| Vollständiger Name | superkiller viralicidic activity 2-like (S. cerevisiae) |
| Berechnetes Molekulargewicht | 1246 aa, 138 kDa |
| Beobachtetes Molekulargewicht | 138 kDa |
| GenBank-Zugangsnummer | BC015758 |
| Gene symbol | SKIV2L |
| Gene ID (NCBI) | 6499 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
The Ski complex is a multiprotein complex required for exosome-mediated RNA surveillance, including the regulation of normal mRNA and the decay of nonfunctional mRNA. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1 complex (PAF1C) [PMID:22444670]. SKIV2L , one component of the Ski complex, acts as an RNA helicase with a role in exosome recruitment or activation. It is involved in the degradation of RNAs by the exosome and may have a role in autophagy [PMID:11719186].
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for SKIV2L antibody 11462-1-AP | Protokoll herunterladen |
| IHC protocol for SKIV2L antibody 11462-1-AP | Protokoll herunterladenl |
| IP protocol for SKIV2L antibody 11462-1-AP | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
J Clin Invest Cytoplasmic RNA quality control failure engages mTORC1-mediated autoinflammatory disease. | ||
Nucleic Acids Res Dom34 mediates targeting of exogenous RNA in the antiviral OAS/RNase L pathway.
| ||
Nucleic Acids Res A short splicing isoform of HBS1L links the cytoplasmic exosome and SKI complexes in humans. | ||
Proc Natl Acad Sci U S A The SKI complex is a broad-spectrum, host-directed antiviral drug target for coronaviruses, influenza, and filoviruses.
|
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Tsimafei (Verified Customer) (08-04-2024) | Antibody stains a band at ~150 kDa. However other non-specific bands are present
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FH Rachel (Verified Customer) (01-31-2019) | This antibody does produce a band at the expected size for SKIV2L, but it also lights up several lower molecular weight, non-specific bands. Additionally, it stains two different ladders that I have run as well.
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