SLAMF6 Polyklonaler Antikörper

SLAMF6 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 22711-1-AP

Synonyme

Activating NK receptor, CD352, KALI, KALIb, Ly108, NK T B antigen, NTB A, NTBA, SF2000, SLAM family member 6, SLAMF6

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  WB analysis of Daudi using 22711-1-AP

  Daudi cells were subjected to SDS PAGE followed by western blot with 22711-1-AP (SLAMF6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 22711-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22711-1-AP (SLAMF6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 22711-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22711-1-AP (SLAMF6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 22711-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22711-1-AP (SLAMF6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 22711-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22711-1-AP (SLAMF6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Daudi cells
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

22711-1-AP bindet in WB, IHC, ELISA SLAMF6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLAMF6 fusion protein Ag18517
• Vollständiger Name: SLAM family member 6
• Berechnetes Molekulargewicht: 332 aa, 37 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC114495
• Gene symbol: SLAMF6
• Gene ID (NCBI): 114836
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Signaling lymphocyte activation molecule 6 (SLAMF6) (Ly108 in mice, NTB-A or SF2000 in humans) is a homophilic receptor belonging to the superfamily immunoglobulin (Ig) domain-containing molecules (PMID:31199820). SLAMF6 is a type I transmembrane protein with two extracellular immunoglobins (Ig)-like domains and three cytoplasmic tyrosine-based signaling motifs, one of which is immunoreceptor tyrosine-based switch motif (PMID:31315913). SLAMF6 is expressed on a wide variety of immune cells including T cells (also TFH), B cells, NK cells (expressed in humans only), double positive thymocytes, eosinophils, and neutrophils (mouse only) (PMID:11862385).