NKCC2 Polyklonaler Antikörper

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• KD/KO Validated

NKCC2 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

25

Kat-Nr. : 18970-1-AP

Synonyme

BSC1, NKCC2, SLC12A1

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 18970-1-AP

  WB result of NKCC2 antibody (18970-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NKCC2 transfected HEK-293 cells.

• 

  WB analysis using 18970-1-AP

  Boiled or non-boiled lysates of mouse kidney were subjected to SDS PAGE followed by western blot with 18970-1-AP (NKCC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 18970-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18970-1-AP (NKCC2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 18970-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18970-1-AP (NKCC2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 18970-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18970-1-AP (NKCC2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 18970-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18970-1-AP (NKCC2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 18970-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 18970-1-AP (NKCC2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 18970-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 18970-1-AP (NKCC2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse Embryonic kidney tissue using 18970-1-AP

  IF rsult of SLC12A1 antibody (18970-1-AP, 1:900) with mouse Embryonic kidneys from E16.5 (fixed with 4% PFA at 4C for 30 min, cryosections 5% goat serum/PBST was used for blocking and antibody incubation). Red: anti-rabbit Alexa555, Blue: Hoechst.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, bei 37°C inkubiertes Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, Mausnierengewebe, Rattennierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: embryonales Mausnierengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:2000-1:8000
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 5 publications below
• IF: See 18 publications below

Produktinformation

18970-1-AP bindet in WB, IHC, IF, ELISA NKCC2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NKCC2 fusion protein Ag5325
• Vollständiger Name: solute carrier family 12 (sodium/potassium/chloride transporters), member 1
• Berechnetes Molekulargewicht: 121 kDa
• Beobachtetes Molekulargewicht: 135 kDa
• GenBank-Zugangsnummer: BC040138
• Gene symbol: SLC12A1
• Gene ID (NCBI): 6557
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human		Cell Stem Cell; Plasticity of distal nephron epithelia from human kidney organoids enables the induction of ureteric tip and stalk.; Authors - Sara E Howden
	IF	Nat Protoc; Generation of proximal tubule-enhanced kidney organoids from human pluripotent stem cells; Authors - Jessica M Vanslambrouck
human	IF	Nat Commun; Enhanced metanephric specification to functional proximal tubule enables toxicity screening and infectious disease modelling in kidney organoids; Authors - Jessica M. Vanslambrouck
human	IF	J Am Soc Nephrol; A Toolbox to Characterize Human Induced Pluripotent Stem Cell-Derived Kidney Cell Types and Organoids.; Authors - Jessica M Vanslambrouck
mouse	IF	J Am Soc Nephrol; Hnf4a Is Required for the Development of Cdh6-Expressing Progenitors into Proximal Tubules in the Mouse Kidney.; Authors - Sierra S Marable
human,mouse	WB,IHC	Mol Ther; Tubular-specific CDK12 knockout causes a defect in urine concentration due to premature cleavage of the slc12a1 gene.; Authors - Bin Wang