SLC13A2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SLC13A2 Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 21722-1-AP

Synonyme

SDCT1, NaDC-1, NADC1, NaDC 1, NaCT

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  IHC staining of human kidney using 21722-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21722-1-AP (SLC13A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 21722-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21722-1-AP (SLC13A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 21722-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21722-1-AP (SLC13A2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Nierengewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 3 publications below

Produktinformation

21722-1-AP bindet in WB, IHC, ELISA SLC13A2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC13A2 fusion protein Ag16379
• Vollständiger Name: solute carrier family 13 (sodium-dependent dicarboxylate transporter), member 2
• Berechnetes Molekulargewicht: 592 aa, 64 kDa
• GenBank-Zugangsnummer: BC096277
• Gene symbol: SLC13A2
• Gene ID (NCBI): 9058
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC13A2 (Solute Carrier Family 13 Member 2) is involved in the transport of citric acid cycle intermediates, such as succinate, citrate, fumarate, and alpha-ketoglutarate (2-oxoglutarate), across cell membranes. In the liver, SLC13A2 has been shown to promote metabolic remodeling by transporting citrate, which is then converted to acetyl-CoA, a precursor for cholesterol synthesis and fatty acid metabolism (PMID: 39824985).

Publikationen

Species	Application	Title
human	WB,IHC	Am J Physiol Renal Physiol; Expression of sodium-dependent dicarboxylate transporter 1 (NaDC1/SLC13A2) in normal and neoplastic human kidney.; Authors - Hyun-Wook Lee
human	IHC	Cancer Med; Transcriptome analysis in primary colorectal cancer tissues from patients with and without liver metastases using next-generation sequencing.; Authors - Sen Wang
mouse		Am J Physiol Renal Physiol; Regulation of Renal NaDC1 Expression and Citrate Excretion by NBCe1-A.; Authors - Gunars Osis
mouse	IHC	Physiol Rep; Effect of NBCe1 deletion on renal citrate and 2-oxoglutarate handling.; Authors - Gunars Osis ; KO Validated