SLC16A5 Polyklonaler Antikörper

SLC16A5 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12120-1-AP

Synonyme

MCT 5, MCT 6, MCT5, MCT6, Monocarboxylate transporter 5, Monocarboxylate transporter 6, SLC16A5

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Galerie der Validierungsdaten

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  WB analysis of mouse skeletal muscle using 12120-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12120-1-AP (SLC16A5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 12120-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12120-1-AP (SLC16A5 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 12120-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12120-1-AP (SLC16A5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 12120-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12120-1-AP (SLC16A5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 12120-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12120-1-AP (SLC16A5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

12120-1-AP bindet in WB, IHC, ELISA SLC16A5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC16A5 fusion protein Ag2762
• Vollständiger Name: solute carrier family 16, member 5 (monocarboxylic acid transporter 6)
• Berechnetes Molekulargewicht: 505 aa, 55 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC009684
• Gene symbol: SLC16A5
• Gene ID (NCBI): 9121
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Monocarboxylate transporter 5 (SLC16A5), also named as MCT6, is a member of the MCT family. SLC16A5 is a proton-linked monocarboxylate transporter that catalyzes the rapid transport across the plasma membrane of many monocarboxylates and it can transport various drugs. It has been reported that SLC16A5 mutation may be a novel genetic risk factor for cisplatin-induced ototoxic (CIO) in testicular cancer patients. There are some isoforms of SLC16A5 among 50-55 kDa. (PMID: 16174808, 28448657)