NPT1 Polyklonaler Antikörper

NPT1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 20751-1-AP

Synonyme

SLC17A1, Na/Pi-4, NAPI 1, Renal Na(+)-dependent phosphate cotransporter 1, Renal sodium-dependent phosphate transport protein 1

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  WB analysis using 20751-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20751-1-AP (NPT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 20751-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 20751-1-AP (NPT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 20751-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20751-1-AP (NPT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Plazenta-Gewebe, Mausnierengewebe, Maus-Skelettmuskelgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below

Produktinformation

20751-1-AP bindet in WB, ELISA NPT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NPT1 fusion protein Ag12819
• Vollständiger Name: solute carrier family 17 (sodium phosphate), member 1
• Berechnetes Molekulargewicht: 467 aa, 51 kDa
• Beobachtetes Molekulargewicht: 50 kDa, 60-64 kDa
• GenBank-Zugangsnummer: BC101745
• Gene symbol: NPT1
• Gene ID (NCBI): 6568
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NPT1 (also known as SLC17A1) is a member of sodium-dependent inorganic phosphate transporters (NPT), which regulates entrance into the cellular membrane. NPT1 is mainly expressed in the kidney transporting small organic anions such as PAH (para-aminohippurate), but it is also found in the liver and brain. NTP1 localizes to the apical membrane of renal proximal tubular cells. NPT1 exits two isoforms due to the alternative splicing. Western blot analysis using this antibody detected a major band around 45-55 kDa in kidney tissue.

Publikationen

Species	Application	Title
human	WB	Exp Mol Med; Impaired Na+-K+-ATPase signaling in renal proximal tubule contributes to hyperuricemia-induced renal tubular injury.; Authors - Jing Xiao
mouse	WB	Phytomedicine; Eurycomanol alleviates hyperuricemia by promoting uric acid excretion and reducing purine synthesis.; Authors - Ruixia Bao
mouse	WB	Am J Physiol Renal Physiol; Transcriptomics of SGLT2-positive early proximal tubule segments in mice: response to type 1 diabetes, SGLT1/2 inhibition, or GLP1 receptor agonism; Authors - Young Chul Kim
mouse	WB	Front Pharmacol; Effect of Eurycoma longifolia Stem Extract on Uric Acid Excretion in Hyperuricemia Mice.; Authors - Ruixia Bao
mouse	WB	Int J Biol Macromol; Diacylglycerol from camellia oil improves hyperuricemia by inhibiting xanthine oxidase and modulating gut microbiota; Authors - Yajuan Chen