SLC17A9 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SLC17A9 Polyklonal Antikörper für WB, IHC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 26731-1-AP

Synonyme

C20orf59, SLC17A9, Vesicular nucleotide transporter, VNUT

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  WB analysis using 26731-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26731-1-AP (SLC17A9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26731-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26731-1-AP (SLC17A9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26731-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26731-1-AP (SLC17A9 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 26731-1-AP

  IP result of anti-SLC17A9 (IP:26731-1-AP, 4ug; Detection:26731-1-AP 1:1000) with mouse brain tissue lysate 4400ug.

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  IHC staining of human skin using 26731-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 26731-1-AP (SLC17A9 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 26731-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 26731-1-AP (SLC17A9 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of human pancreas using 26731-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human pancreas tissue using 26731-1-AP (SLC17A9 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human pancreas using 26731-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human pancreas tissue using 26731-1-AP (SLC17A9 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhirngewebe, C2C12-Zellen, Caco-2-Zellen, fetales humanes Hirngewebe, Maushirngewebe, THP-1-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Pankreasgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

26731-1-AP bindet in WB, IHC, IF-P, IP, ELISA SLC17A9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC17A9 fusion protein Ag24970
• Vollständiger Name: solute carrier family 17, member 9
• Berechnetes Molekulargewicht: 436 aa, 47 kDa
• Beobachtetes Molekulargewicht: 68-70 kDa
• GenBank-Zugangsnummer: BC025312
• Gene symbol: SLC17A9
• Gene ID (NCBI): 63910
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IHC,WB	iScience; SLC17A9-PTHLH-EMT axis promotes proliferation and invasion of clear renal cell carcinoma; Authors - Weiquan Li ; KD Validated
human	IHC	bioRxiv; Optimization of protocols for immunohistochemical assessment of enteric nervous system in formalin fixed human tissue; Authors - Su Min Hong
human,mouse	IHC,IF	iScience; A purinergic mechanism underlying metformin regulation of hyperglycemia; Authors - Jared Senfeld
human	WB	Oncol Lett; SLC17A9 expression levels in a pan‑cancer panel and validation of the role of SLC17A9 as a novel prognostic biomarker for osteosarcoma; Authors - Junqing Li ; KD Validated