SLC22A2 Polyklonaler Antikörper

SLC22A2 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 13594-1-AP

Synonyme

hOCT2, OCT2, Organic cation transporter 2, SLC22A2

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Galerie der Validierungsdaten

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  WB analysis using 13594-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13594-1-AP (SLC22A2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13594-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13594-1-AP (SLC22A2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, HEK-293-Zellen, Rattennierengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

13594-1-AP bindet in WB, ELISA SLC22A2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC22A2 fusion protein Ag4500
• Vollständiger Name: solute carrier family 22 (organic cation transporter), member 2
• Berechnetes Molekulargewicht: 555 aa, 63 kDa
• Beobachtetes Molekulargewicht: 60-70 kDa
• GenBank-Zugangsnummer: BC039899
• Gene symbol: SLC22A2
• Gene ID (NCBI): 6582
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC22A2, also named as OCT2, belongs to the major facilitator (TC 2.A.1) superfamily and Organic cation transporter (TC 2.A.1.19) family. The SLC22A2 gene encodes for the organic cation transporter 2 (OCT2) protein which acts as a renal uptake transporter. Human OCT2 (hOCT2) is expressed on the basolateral side of the proximal tubule cells and plays a crucial role in the disposition and renal clearance of cationic drugs and endogenous compounds (PMID: 35143948). SLC22A2 has 3 isoforms with the molecular mass of 27, 54 and 63 kDa. Sometimes higher molecular weight around 70 kDa can also be observed, which may be a modified variant of SLC22A2.

Publikationen

Species	Application	Title
human	WB	Biomed Pharmacother; Organic cation transporter 2 activation enhances sensitivity to oxaliplatin in human pancreatic ductal adenocarcinoma; Authors - Ching-Feng Chiu
human,mouse	WB	J Pharm Anal; Discovering metabolic vulnerability using spatially resolved metabolomics for antitumor small molecule-drug conjugates development as a precise cancer therapy strategy; Authors - Xiangyi Wang