OCTN2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

OCTN2 Polyklonal Antikörper für FC, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 16331-1-AP

Synonyme

CDSP, OCTN2, OCTN2VT, SLC22A5

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Galerie der Validierungsdaten

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  WB analysis of mouse small intestine using 16331-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 16331-1-AP (OCTN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 16331-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 16331-1-AP (OCTN2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 16331-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16331-1-AP (OCTN2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 16331-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16331-1-AP (OCTN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 16331-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 16331-1-AP (OCTN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 16331-1-AP

  IP Result of anti-OCTN2 (IP:16331-1-AP, 4ug; Detection:16331-1-AP 1:500) with mouse skeletal muscle tissue lysate 2200ug.

• 

  IHC staining of human kidney using 16331-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16331-1-AP (OCTN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16331-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16331-1-AP (OCTN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HEK-293 using 16331-1-AP

  1X10^6 HEK-293 cells were stained with 0.2ug OCTN2 antibody (16331-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Dünndarmgewebe, humanes Plazenta-Gewebe, Maushodengewebe, Maus-Pankreasgewebe, Maus-Skelettmuskelgewebe
• Erfolgreiche IP: Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below

Produktinformation

16331-1-AP bindet in WB, IP, IHC, FC, ELISA OCTN2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OCTN2 fusion protein Ag9450
• Vollständiger Name: solute carrier family 22 (organic cation/carnitine transporter), member 5
• Berechnetes Molekulargewicht: 557 aa, 63 kDa
• Beobachtetes Molekulargewicht: 70-80 kDa
• GenBank-Zugangsnummer: BC012325
• Gene symbol: SLC22A5
• Gene ID (NCBI): 6584
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OCTN2 (Organic Cation/Carnitine Transporter 2), encoded by SLC22A5, is a ubiquitously expressed organic anion/cation transporter. OCTN2 plays a key role in absorption, tissue distribution and renal reabsorption of L-carnitine which is an essential cofactor in fat metabolism. Defects in the OCTN2 are responsible for primary carnitine deficiency. OCTN2 also transports some important respiratory medicines (such as tiotropium), and due to its expression in the lung, may influence the disposition and absorption of these medicines in the lung.

Publikationen

Species	Application	Title
mouse	WB	Cell Metab; Global Analysis of Plasma Lipids Identifies Liver-Derived Acylcarnitines as a Fuel Source for Brown Fat Thermogenesis.; Authors - Judith Simcox
rat	WB	Food Funct; Ferulic acid supplementation alleviates hyperuricemia in high-fructose/fat diet-fed rats via promoting uric acid excretion and mediating the gut microbiota; Authors - Nanhai Zhang
mouse	WB	Nutrients; The Enhancement of Acylcarnitine Metabolism by 5-Heptadecylresorcinol in Brown Adipose Tissue Contributes to Improving Glucose and Lipid Levels in Aging Male Mice; Authors - Kuiliang Zhang
rat	WB	Front Pharmacol; L-carnitine prevents lenvatinib-induced muscle toxicity without impairment of the anti-angiogenic efficacy; Authors - Zheng Jing
human	WB	J Neurochem; Identification of drug transporters contributing to oxaliplatin-induced peripheral neuropathy.; Authors - S Fujita ; KD Validated
rat	WB	Toxicol Lett; Lenvatinib causes reduced expression of carnitine/organic cation transporter 2 and carnitine deficiency in the skeletal muscle of rats.; Authors - Zheng Jing