SLC24A4 Polyklonaler Antikörper

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• KD/KO Validated

SLC24A4 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 18992-1-AP

Synonyme

NCKX4, SHEP6, SLC24A2, SLC24A4

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Galerie der Validierungsdaten

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  WB analysis using 18992-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18992-1-AP (SLC24A4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa cells using 18992-1-AP

  WB result of SLC24A4 antibody (18992-1-AP, 1:1000) with si-Control and si-SLC24A4 transfected HeLa cells.

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  WB analysis of mouse brain using 18992-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18992-1-AP (SLC24A4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 18992-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18992-1-AP (SLC24A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 18992-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18992-1-AP (SLC24A4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18992-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18992-1-AP (SLC24A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18992-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18992-1-AP (SLC24A4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 18992-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18992-1-AP (SLC24A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 18992-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18992-1-AP (SLC24A4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse eye using 18992-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using SLC24A4 antibody (18992-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse eye using 18992-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using SLC24A4 antibody (18992-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HeLa-Zellen, Mausherzgewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maus-Augengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

18992-1-AP bindet in WB, IHC, IF, ELISA SLC24A4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: solute carrier family 24 (sodium/potassium/calcium exchanger), member 4
• Berechnetes Molekulargewicht: 69 kDa
• Beobachtetes Molekulargewicht: 62-90 kDa
• GenBank-Zugangsnummer: NM_153646
• Gene symbol: SLC24A4
• Gene ID (NCBI): 123041
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC24A4, also named as NCKX4, belongs to the sodium/potassium/calcium exchanger family. SLC24A4 transports 1 Ca2+ and 1 K+ in exchange for 4 Na+. Genetic variations in SLC24A4 are associated with skin/hair/eye pigmentation type 6 (SHEP6).

Publikationen

Species	Application	Title
mouse	IHC	Bone; Deletion of epithelial cell-specific p130Cas impairs the maturation stage of amelogenesis.; Authors - Akane Inoue
rat	WB	J Cell Physiol; Identification of novel candidate genes involved in mineralization of dental enamel by genome-wide transcript profiling.; Authors - Lacruz Rodrigo S RS
mouse	WB	Calcif Tissue Int; Reduced Protein Expression of the Na(+)/Ca(2+)+K(+)-Exchanger (SLC24A4) in Apical Plasma Membranes of Maturation Ameloblasts of Fluorotic Mice.; Authors - A L J J Bronckers
mouse	WB	Cells Tissues Organs; Expression of the sodium/calcium/potassium exchanger, NCKX4, in ameloblasts.; Authors - Hu Ping P