SLC25A10 Polyklonaler Antikörper

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• KD/KO Validated

SLC25A10 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 12086-1-AP

Synonyme

DIC, SLC25A10

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Galerie der Validierungsdaten

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  WB analysis using 12086-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12086-1-AP (SLC25A10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 12086-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12086-1-AP (SLC25A10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 12086-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12086-1-AP (SLC25A10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 12086-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12086-1-AP (SLC25A10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 12086-1-AP

  IP Result of anti-SLC25A10 (IP:12086-1-AP, 3ug; Detection:12086-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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  IHC staining of human lung using 12086-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12086-1-AP (SLC25A10 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 12086-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12086-1-AP (SLC25A10 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 12086-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12086-1-AP (SLC25A10 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 12086-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12086-1-AP (SLC25A10 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A549-Zellen, HepG2-Zellen, Mauslebergewebe, Mausnierengewebe, PC-3-Zellen
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Lungengewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below

Produktinformation

12086-1-AP bindet in WB, IP, IHC, ELISA SLC25A10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC25A10 fusion protein Ag2719
• Vollständiger Name: solute carrier family 25 (mitochondrial carrier; dicarboxylate transporter), member 10
• Berechnetes Molekulargewicht: 296 aa, 32 kDa
• Beobachtetes Molekulargewicht: 29-31 kDa
• GenBank-Zugangsnummer: BC015797
• Gene symbol: SLC25A10
• Gene ID (NCBI): 1468
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC25A10, also known as dicarboxylate ion carrier (DIC), is a mitochondrial inner membrane carrier protein which catalyzes the transport of dicarboxylates such as malate and succinate across the mitochondrial membrane in exchange for phosphate, sulfate, and thiosulfate. SLC25A10 is expressed at highest levels in kidney and liver, lower levels in lung, spleen, heart, and brain, and lowest levels in skeletal muscle. Two isoforms of SLC25A10 exist due to the alternative splicing.

Publikationen

Species	Application	Title
human	IHC,WB	Hum Cell; The role of PYCR1 in inhibiting 5-fluorouracil-induced ferroptosis and apoptosis through SLC25A10 in colorectal cancer; Authors - Borong Zhou
human	WB,IHC	Oncol Lett; SLC25A10 performs an oncogenic role in human osteosarcoma.; Authors - Gaoyuan Wang ; KD Validated