SLC25A13 Polyklonaler Antikörper

SLC25A13 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 10789-1-AP

Synonyme

Mitochondrial aspartate glutamate carrier 2, Electrogenic aspartate/glutamate antiporter SLC25A13, mitochondrial, Citrin, Calcium-binding mitochondrial carrier protein Aralar2, ARALAR-related gene 2

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  WB analysis of mouse kidney using 10789-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10789-1-AP (SLC25A13 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis using 10789-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10789-1-AP (SLC25A13 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10789-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10789-1-AP (SLC25A13 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 10789-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10789-1-AP (SLC25A13 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10789-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10789-1-AP (SLC25A13 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10789-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10789-1-AP (SLC25A13 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 10789-1-AP

  IP result of anti-SLC25A13 (IP:10789-1-AP, 4ug; Detection:10789-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human colon cancer using 10789-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10789-1-AP (SLC25A13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10789-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10789-1-AP (SLC25A13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 10789-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SLC25A13 antibody (10789-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, A431-Zellen, HEK-293-Zellen, Mausnierengewebe, Mauslebergewebe, Maus-Eierstockgewebe, Raji-Zellen
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

10789-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA SLC25A13 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC25A13 fusion protein Ag1242
• Vollständiger Name: solute carrier family 25, member 13 (citrin)
• Berechnetes Molekulargewicht: 74 kDa
• Beobachtetes Molekulargewicht: 60-63 kDa
• GenBank-Zugangsnummer: BC006566
• Gene symbol: SLC25A13
• Gene ID (NCBI): 10165
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC25A13, also known as citrin, has been identified as a mitochondrial aspartate-glutamate carrier protein and may be implicated in metabolic compartmentation. It consists of 675 amino acid residues and harbors four EF-hands and six mitochondrial transmembranous (TM) spanners. Mutations in SLC25A13 gene cause adult-onset type II citrullinemia (CTLN2) and neonatal intrahepatic cholestasis (NICCD). The complete deletion or cleaved SLA25A13 proteins have been reported in patients with SLC25A13 mutation.

Publikationen

Species	Application	Title
human	WB	Cancer Cell Int; Unveiling the oncogenic role of SLC25A13: a multi-omics pan-cancer analysis reveals its impact on glioma progression; Authors - Wenjie Wu