SLC25A23 Polyklonaler Antikörper

SLC25A23 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 20168-1-AP

Synonyme

APC2, MCSC2, Mitochondrial adenyl nucleotide antiporter SLC25A23, Mitochondrial ATP-Mg/Pi carrier protein 2, SCaMC 3

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  WB analysis of mouse pancreas using 20168-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 20168-1-AP (SLC25A23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 20168-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20168-1-AP (SLC25A23 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 20168-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20168-1-AP (SLC25A23 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 20168-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20168-1-AP (SLC25A23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Pankreasgewebe, Maus-Skelettmuskelgewebe, Maushodengewebe, SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

20168-1-AP bindet in WB, IHC, ELISA SLC25A23 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC25A23 fusion protein Ag13987
• Vollständiger Name: solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 23
• Berechnetes Molekulargewicht: 468 aa, 52 kDa
• Beobachtetes Molekulargewicht: 49-54 kDa
• GenBank-Zugangsnummer: BC001656
• Gene symbol: SLC25A23
• Gene ID (NCBI): 79085
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC25A23 gene encodes a 468 amino acids polypeptide, named SCaMC-3, with a bipartite structure typical of calcium-binding mitochondrial solute carrier (CaMSC) proteins. The amino-terminal portion harbors three canonical EF-hand calcium-binding domains while the carboxyl-terminal portion of SCaMC-3 has the characteristic features of the mitochondrial solute carrier (MSC) superfamily. Northern blot analysis reveals the presence of the transcript in brain, heart, skeletal muscle, liver and small intestine. The SLC25A23 gene undergoes alternative splicing suggesting a modular nature of the encoded product. Three out of four putative protein isoforms lack a significant portion of the third mitochondrial carrier signature. The most common SCaMC-3 isoform shows a mitochondrial subcellular localization when transfected in HeLa cells and is able to bind calcium by Ca2+ dependent mobility shift assays.(PMID: 15716113)

Publikationen

Species	Application	Title
human	IHC	Biomed Res Int; Comprehensive Analysis and Validation of Solute Carrier Family 25 (SLC25) and Its Correlation with Immune Infiltration in Pan-Cancer; Authors - Ao-ran Liu