ANT1/2 Polyklonaler Antikörper

ANT1/2 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 17796-1-AP

Synonyme

2F1, AAC2, ADP,ATP carrier protein 2, ADP/ATP translocase 2, ANT 2, ANT1/2, SLC25A5, T2, T3

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Galerie der Validierungsdaten

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  WB analysis using 17796-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17796-1-AP (ANT1/2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 17796-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17796-1-AP (ANT1/2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 17796-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17796-1-AP (ANT1/2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 17796-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17796-1-AP (ANT1/2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 17796-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17796-1-AP (ANT1/2 antibody) at dilution of 1:500 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 17796-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17796-1-AP (ANT1/2 antibody) at dilution of 1:500 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 17796-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17796-1-AP (ANT1/2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 17796-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17796-1-AP (ANT1/2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HepG2-Zellen, Mausherzgewebe, Maushirngewebe, Mauslebergewebe, Mausnierengewebe, Rattenherzgewebe, Rattenhirngewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Leberkarzinomgewebe, humanes Magenkrebsgewebe, humanes Zervixkarzinomgewebe, Mausherzgewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IHC: See 5 publications below

Produktinformation

17796-1-AP bindet in WB, IHC, ELISA ANT1/2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ANT1/2 fusion protein Ag11956
• Vollständiger Name: solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 5
• Berechnetes Molekulargewicht: 298 aa, 33 kDa
• Beobachtetes Molekulargewicht: 30-32 kDa, 60 kDa
• GenBank-Zugangsnummer: BC056160
• Gene symbol: SLC25A5
• Gene ID (NCBI): 292
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Adenine nucleotide translocators (ANTs) are mitochondrial carrier proteins that exchange ATP and ADP between the cytoplasm and the mitochondrial matrix. Four isoforms of ANT were identified: ANT1 (encoded by SLC25A4) is highly expressed in different tissues; ANT2 (encoded by SLC25A5) is predominantly expressed in proliferating, regenerating, or undifferentiated cells; ANT3 is ubiquitously expressed; ANT4 is mainly expressed in testis and germ cells. This antibody recognizes both of ANT1 and ANT2. The predicted molecular weight of ANT1/2 protein is around 32 kDa. While 60 kDa protein can also be observed, which has been proposed as the dimer of ANT1/2. (22423112)

Publikationen

Species	Application	Title
	WB	Elife; Legionella pneumophila modulates host energy metabolism by ADP-ribosylation of ADP/ATP translocases.; Authors - Jiaqi Fu
mouse	WB	PLoS Genet; Novel insights into SLC25A46-related pathologies in a genetic mouse model.; Authors - Maria Eirini Terzenidou
human	IHC	Mol Cell Proteomics; Proteomics of skin proteins in psoriasis: from discovery and verification in a mouse model to confirmation in humans.; Authors - Kathleen C Lundberg
rat	WB	Exp Neurol; Physical exercise ameliorates psychiatric disorders and cognitive dysfunctions by hippocampal mitochondrial function and neuroplasticity in post-traumatic stress disorder.; Authors - Jin-Hee Seo
mouse	WB	J Proteome Res; Proteomic Identification of the SLC25A46 Interactome in Transgenic Mice Expressing SLC25A46-FLAG.; Authors - Vasiliki-Iris Perivolidi
human	WB	Biology (Basel); TERT Promoter Revertant Mutation Inhibits Melanoma Growth through Intrinsic Apoptosis.; Authors - Yanbing Wang