SLC25A6-Specific Polyklonaler Antikörper

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• KD/KO Validated

SLC25A6-Specific Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 14841-1-AP

Synonyme

SLC25A6, ANT 3, ANT 2, ADP/ATP translocase 3, N-terminally processed, ADP/ATP translocase 3

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  WB analysis using 14841-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14841-1-AP

  WB result of SLC25A6-Specific antibody (14841-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLC25A6-Specific transfected HEK-293 cells.

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  WB analysis of HEK-293 using 14841-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 14841-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 14841-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 14841-1-AP

  IP result of anti-SLC25A6-Specific (IP:14841-1-AP, 4ug; Detection:14841-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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  IHC staining of human skeletal muscle using 14841-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skeletal muscle using 14841-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 14841-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14841-1-AP (SLC25A6-Specific Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 14841-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14841-1-AP (SLC25A6-Specific Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 14841-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14841-1-AP (SLC25A6-Specific Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 14841-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14841-1-AP (SLC25A6-Specific Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human heart using 14841-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 14841-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 14841-1-AP

  Immunofluorescent analysis of HepG2 cells using 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  IF Staining of MCF-7 using 14841-1-AP

  Immunofluorescent analysis of MCF-7 cells using 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, HEK-293-Zellen, HEK-293T-Zellen, humanes Herzgewebe, humanes Lebergewebe, humanes Plazenta-Gewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Skelettmuskelgewebe, humanes Herzgewebe, humanes Nierengewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

14841-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA SLC25A6-Specific und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 6
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 30-33 kDa
• GenBank-Zugangsnummer: NM_001636
• Gene symbol: SLC25A6
• Gene ID (NCBI): 293
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC25A6, also known as ANT3 (adenine nucleotide translocase 3), is a member of the solute carrier family 25, specifically the mitochondrial carrier subfamily. As part of the mitochondrial carrier family, SLC25A6 plays a role in the mitochondrial energy production process by facilitating the exchange of metabolites necessary for oxidative phosphorylation (PMID: 23506884).

Publikationen

Species	Application	Title
human	WB	Curr Biol; Defining the Substrate Spectrum of the TIM22 Complex Identifies Pyruvate Carrier Subunits as Unconventional Cargos.; Authors - Ridhima Gomkale
human	WB,CoIP	Am J Cancer Res; PTPMT1 regulates mitochondrial death through the SLC25A6-NDUFS2 axis in pancreatic cancer cells; Authors - Peng-Peng Ding
	WB	Hum Mol Genet; Mutations of the mitochondrial carrier translocase channel subunit TIM22 cause early-onset mitochondrial myopathy.; Authors - David Pacheu-Grau
human	WB,IF	Cancer Cell Int; Discovery and validation of FBLN1 and ANT3 as potential biomarkers for early detection of cervical cancer.; Authors - Yi Hao
human	WB	FEBS Lett; TIM29 is a subunit of the human carrier translocase required for protein transport.; Authors - Sylvie Callegari
human	WB,IHC,CoIP	iScience; MRPL12-ANT3 interaction involves in acute kidney injury via regulating MPTP of tubular epithelial cells; Authors - Xingzhao Ji