FATP2 Polyklonaler Antikörper

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• KD/KO Validated

FATP2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(48)

Kat-Nr. : 14048-1-AP

Synonyme

SLC27A2, EC:6.2.1.3, EC:6.2.1.24, EC:6.2.1.15, EC:6.2.1.-

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  WB analysis using 14048-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14048-1-AP (FATP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14048-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14048-1-AP (FATP2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 14048-1-AP

  IP result of anti-FATP2 (IP:14048-1-AP, 4ug; Detection:14048-1-AP 1:500) with HepG2 cells lysate 3200ug.

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  IHC staining of human kidney using 14048-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14048-1-AP (FATP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 14048-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14048-1-AP (FATP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14048-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14048-1-AP (FATP2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14048-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14048-1-AP (FATP2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 14048-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FATP2 antibody (14048-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 42 publications below
• IHC: See 5 publications below
• IF: See 7 publications below
• CoIP: See 1 publications below

Produktinformation

14048-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA FATP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FATP2 fusion protein Ag5217
• Vollständiger Name: solute carrier family 27 (fatty acid transporter), member 2
• Berechnetes Molekulargewicht: 567 aa, 65 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC057770
• Gene symbol: FATP2/SLC27A2
• Gene ID (NCBI): 11001
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FATP2 is a member of the FATP family which functions in lipid and bile metabolism. It is a 70-kDa protein predominantly expressed in liver and kidney. Kidney FATP2 is localized exclusively to proximal tubule epithelial cells along the apical but not the basolateral membrane, and regulates lipoapoptosis. FATP2 is involved in metabolism-related diseases including nonalcoholic fatty liver disease (NAFLD) and type 2 diabetes mellitus (T2DM), and is a potential clinical biomarker and therapeutic target.

Publikationen

Species	Application	Title
mouse	WB	Hepatology; Letter to the editor: Adipose lipolysis is important for ethanol to induce fatty liver in the NIAAA murine model of chronic and binge ethanol feeding; Authors - Wenhao Luo
mouse	WB	Hepatology; Adipose lipolysis is important for ethanol to induce fatty liver in the National Institute on Alcohol Abuse and Alcoholism murine model of chronic and binge ethanol feeding.; Authors - Mallika Mathur
rat	WB	Environ Pollut; Hepatotoxicity induced in rats by chronic exposure to F-53B, an emerging replacement of perfluorooctane sulfonate (PFOS); Authors - Xiaohan Li
human	WB	EMBO Rep; Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.; Authors - Yang Zhang
human,mouse	WB,IHC	Biomed Pharmacother; Inhibition of ATP-citrate lyase by bempedoic acid protects against abdominal aortic aneurysm formation in mice; Authors - Lídia Puertas-Umbert
human	WB	Anal Chem; Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.; Authors - Hongwei Chu