GLUT1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GLUT1 Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (3)

Anwendung

WB, IHC, IF, FC, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

235

Rezensionen (5)

Kat-Nr. : 21829-1-AP

Synonyme

DYT17, DYT18, GLUT, GLUT 1, GLUT1, HepG2 glucose transporter, PED, SLC2A1

---

Galerie der Validierungsdaten

• 

  WB analysis using 21829-1-AP

  HT-29 cell lysates (boiled or unboiled) were subjected to SDS PAGE followed by western blot with 21829-1-AP (GLUT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21829-1-AP

  Mouse colon lysates (boiled or unboiled) were subjected to SDS PAGE followed by western blot with 21829-1-AP (GLUT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21829-1-AP

  Mouse brain lysates (boiled or unboiled) were subjected to SDS PAGE followed by western blot with 21829-1-AP (GLUT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 21829-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21829-1-AP (GLUT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 21829-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21829-1-AP (GLUT1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 21829-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21829-1-AP (GLUT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 21829-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21829-1-AP (GLUT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 21829-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GLUT1 antibody (21829-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 21829-1-AP

  1X10^6 HeLa cells were stained with 0.2ug SLC2A1,GLUT1 antibody (21829-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-29-Zellen, bei 37°C inkubiertes Maus-Kolongewebe
• Erfolgreiche Detektion in IHC: Rattenhirngewebe, humanes Lungenkarzinomgewebe, humanes Mammakarzinomgewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:2500-1:10000
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 190 publications below
• IHC: See 36 publications below
• IF: See 35 publications below
• FC: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

21829-1-AP bindet in WB, IHC, IF, FC, ChIP, ELISA GLUT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte, Ziege, Brandts Mongolische Wühlmaus (Lasiopodomys brandtii)
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GLUT1 fusion protein Ag16282
• Vollständiger Name: solute carrier family 2 (facilitated glucose transporter), member 1
• Berechnetes Molekulargewicht: 492 aa, 54 kDa
• Beobachtetes Molekulargewicht: 45-55 kDa
• GenBank-Zugangsnummer: BC121804
• Gene symbol: SLC2A1
• Gene ID (NCBI): 6513
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glucose transporter 1 (GLUT1), also known as solute carrier family 2, facilitated glucose transporter member 1 (SLC2A1), is a uniporter protein responsible for the transport of glucose in many cell types and across the blood-brain barrier.

What is the molecular weight of GLUT1? Is GLUT1 post-translationally modified?

There are two forms of GLUT1 transporter that differ in their molecular weight. The 45-kDa form is found in glial cells, while the 55-kDa form is present in the endothelial cells regulating glucose transport over the blood-brain and blood-tissue barriers (PMID: 9630522). N-glycosylation of asparagine at position 42 is the only known post-translation modification of GLUT1 (PMID: 3839598).

What is the subcellular localization of GLUT1?

Glucose transporters, including GLUT1, are multiple-pass integral membrane proteins. GLUT1 is present at the plasma membrane but is also a subject of recycling between plasma membrane and endosomes.

What molecules can be transported by GLUT1?

The main substrate of GLUT1 transport is glucose, but it can also transport galactose, mannose, glucosamine, and reduced ascorbate.

What is the tissue expression pattern of GLUT1?

GLUT1 is expressed by many cell types but the highest levels are observed in erythrocytes and in the central nervous system (astrocytes). GLUT1 is responsible for glucose transfer across the blood-brain and blood-tissue barriers, including placental transport.

Publikationen

Species	Application	Title
human	WB	Cancer Cell; Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.; Authors - Ling Li
mouse	WB,IHC	Int J Oral Sci; Transcriptional activation of glucose transporter 1 in orthodontic tooth movement-associated mechanical response.; Authors - Yu Wang
human	WB	Nat Commun; Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.; Authors - Xiaoyu Ma
rat	WB	Redox Biol; Pharmaceutical targeting of succinate dehydrogenase in fibroblasts controls bleomycin-induced lung fibrosis.; Authors - Ziwen Wang
mouse	WB	EMBO J; Asparagine reinforces mTORC1 signaling to boost thermogenesis and glycolysis in adipose tissues.; Authors - Yingjiang Xu
	WB	Sci Total Environ; Metastatic effects of perfluorooctanoic acid (PFOA) on Drosophila melanogaster with metabolic reprogramming and dysrhythmia in a multigenerational exposure scenario; Authors - Fangnon Firmin Fangninou

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Hua (Verified Customer) (02-14-2023)

Good antibody working for WB with mouse liver samples. However, boiling mouse colon and brain tissues results in no band observed.

• Applications: Western Blot
• Primary Antibody Dilution: 1:4000
• Cell Tissue Type: mouse liver, brain, and colon

GLUT1 Antibody Western Blot validation (1:4000 dilution) in mouse liver, brain, and colon (Cat no:21829-1-AP)

FH

William (Verified Customer) (10-26-2021)

Very clear staining in IHC in rat brain tissue (Wistar) at a concentration of 1:100, very well localised to blood vessels. Not tested yet at different concentrations

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Rat brain

FH

Bastien (Verified Customer) (08-19-2020)

Staining of B cells from mice bone marrow after cytoplasmic permezbiliation. The cells were first fixed and permeabilized with intracellular fix and perm set from ebioscience and then stained with 1 µl/ million cells with Glut-1 antibody (21829-1-AP) during 50min After, a seconde staining was performed with 0,1µl/million cells of F(ab')2-Donkey anti-Rabbit IgG (H+L), PE, Secondary Antibody from invitrogen. In blue secondary antibody alone and in red primary (21829-1-AP) +secondary antibody

• Applications: Flow Cytometry
• Primary Antibody Dilution: 1µl/10^6cells
• Cell Tissue Type: B cells

GLUT1 Antibody Flow Cytometry validation (1µl/10^6cells dilution) in B cells (Cat no:21829-1-AP)

FH

Susan (Verified Customer) (11-19-2019)

20ug of HeLa cells overexpressing Glut1-GFP. Blocked with 5% milk in 0.1% TBST and incubated overnight at 4 degrees with rocking.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HeLa

GLUT1 Antibody Western Blot,Immunofluorescence, validation (1:2000 dilution) in HeLa (Cat no:21829-1-AP)

FH

Kishor (Verified Customer) (12-13-2018)

I got good results in cultured cells but I could not see any results with rat liver protein.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: H69, HUCCT-1

Verwandte Produkte

GLUT3 Polyclonal antibody

20403-1-AP

GLUT4 Polyclonal antibody

21048-1-AP

Hexokinase 2 Polyclonal antibody

22029-1-AP

LDHA-Specific Polyclonal antibody

19987-1-AP

HIF-1 alpha Polyclonal antibody

20960-1-AP