SLC30A2 Monoklonaler Antikörper

SLC30A2 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

1D9E5

Kat-Nr. : 67993-1-Ig

Synonyme

FLJ36708, PP12488, SLC30A2, Zinc transporter 2, ZnT 2, ZNT2

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  WB analysis using 67993-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67993-1-Ig (SLC30A2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 67993-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67993-1-Ig (SLC30A2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 67993-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67993-1-Ig (SLC30A2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, ATDC-5-Zellen, Caco-2-Zellen, HaCaT-Zellen, HeLa-Zellen, HT-29-Zellen, JAR-Zellen, NCCIT-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

67993-1-Ig bindet in WB, IHC, IF, ELISA SLC30A2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SLC30A2 fusion protein Ag8232
• Vollständiger Name: solute carrier family 30 (zinc transporter), member 2
• Berechnetes Molekulargewicht: 323 aa, 35 kDa
• Beobachtetes Molekulargewicht: 41 kDa
• GenBank-Zugangsnummer: BC006251
• Gene symbol: SLC30A2
• Gene ID (NCBI): 7780
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC30A2, also named as the Zn transporter 2 gene (ZnT2), belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family and SLC30A subfamily. It plays a role in the zinc ion transmembrane transport. Expression of SLC30A2 is restricted to secretory cells, such as acinar pancreatic cells, prostate epithelial cells, placental trophoblasts, Paneth cells, and mammary epithelial cells (MECs). SLC30A2 consists of six transmembrane domains with cytoplasmic N- and C-termini that contain numerous regulatory domains, and functions as a homo- or hetero- dimer to transport Zn into vesicles (PMID: 29476070 ). SLC30A2 has 2 isoforms with the molecular mass of 35 and 41 kDa.

Publikationen

Species	Application	Title
human,mouse	WB	Sci Adv; Bimetallic peroxide nanoparticles induce PANoptosis by disrupting ion homeostasis for enhanced immunotherapy; Authors - Guanghui Hou
mouse	IF	iScience; Different populations of mouse egg cortical vesicles are responsible for post-fertilization zinc sparks and proteolysis of the zona pellucida; Authors - Omar José