SLC30A8/ZNT8-Specific Polyklonaler Antikörper

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• KD/KO Validated

SLC30A8/ZNT8-Specific Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 16169-1-AP

Synonyme

SLC30A8, SLC30A8/ZNT8-Specific, Zinc transporter 8, ZnT 8, ZNT8

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Galerie der Validierungsdaten

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  WB analysis of mouse pancreas using 16169-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 16169-1-AP (SLC30A8/ZNT8-Specific antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 16169-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 16169-1-AP (SLC30A8/ZNT8-Specific antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 16169-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 16169-1-AP (SLC30A8/ZNT8-Specific antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 16169-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16169-1-AP (SLC30A8/ZNT8-Specific antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 16169-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16169-1-AP (SLC30A8/ZNT8-Specific antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16169-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16169-1-AP (SLC30A8/ZNT8-Specific antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 16169-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16169-1-AP (SLC30A8/ZNT8-Specific antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of human pancreas using 16169-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human pancreas tissue using 16169-1-AP (SLC30A8/ZNT8-Specific antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Red: 66198-1-Ig (Insulin antibody).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Pankreasgewebe
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe, humanes Leberkarzinomgewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Pankreasgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

16169-1-AP bindet in WB, IHC, IF, ELISA SLC30A8/ZNT8-Specific und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: solute carrier family 30 (zinc transporter), member 8
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: NM_173851
• Gene symbol: SLC30A8
• Gene ID (NCBI): 169026
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC30A8 (also named as ZNT8) encods the secretory granule zinc transporter, permiting cellular efflux of zinc. SLC30A8 was recently defined as a autoantigen candidates of type 2 diabetes, although the molecular mechanism through which this phenotype is manifest is incompletely understood.

Publikationen

Species	Application	Title
human	WB	J Biol Chem; Lipid-tuned Zinc Transport Activity of Human ZnT8 Correlates with Risk for Type-2 Diabetes.; Authors - Chengfeng Merriman
rat	WB	J Biol Chem; PMID: 28130446; Authors - Qiong Huang
mouse	WB	J Biol Chem; A subclass of serum anti-ZnT8 antibodies directed to the surface of live pancreatic β-cells.; Authors - Chengfeng Merriman
		J Biol Chem; Highly specific monoclonal antibodies for allosteric inhibition and immunodetection of the human pancreatic zinc transporter ZnT8.; Authors - Chengfeng Merriman
human	WB	J Endocrinol; Lack of ZnT8 protects pancreatic islets from hypoxia- and cytokine induced cell death.; Authors - Maria Karsai
human	IHC	Sci Rep; A change in the zinc ion concentration reflects the maturation of insulin-producing cells generated from adipose-derived mesenchymal stem cells.; Authors - Shogo Ohta

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