SLC35A2 Polyklonaler Antikörper

SLC35A2 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 13657-1-AP

Synonyme

SLC35A2, UDP Gal Tr, UDP galactose translocator, UDP galactose transporter, UGALT, UGAT, UGT, UGT1, UGT2, UGTL

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 13657-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13657-1-AP (SLC35A2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13657-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13657-1-AP (SLC35A2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 13657-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13657-1-AP (SLC35A2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 13657-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13657-1-AP (SLC35A2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 13657-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13657-1-AP (SLC35A2 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 13657-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13657-1-AP (SLC35A2 antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 4 publications below

Produktinformation

13657-1-AP bindet in WB, IHC, ELISA SLC35A2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC35A2 fusion protein Ag4524
• Vollständiger Name: solute carrier family 35 (UDP-galactose transporter), member A2
• Berechnetes Molekulargewicht: 396 aa, 41 kDa
• Beobachtetes Molekulargewicht: 41 kDa, 32 kDa
• GenBank-Zugangsnummer: BC035747
• Gene symbol: SLC35A2
• Gene ID (NCBI): 7355
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IHC,WB	Cell Oncol (Dordr); Nucleotide sugar transporter SLC35A2 is involved in promoting hepatocellular carcinoma metastasis by regulating cellular glycosylation; Authors - Hongxia Cheng ; KD Validated
	IHC	Front Immunol; Comprehensive analysis of 33 human cancers reveals clinical implications and immunotherapeutic value of the solute carrier family 35 member A2; Authors - Shengshan Xu
human	IHC,WB	Aging (Albany NY); Comprehensive analysis of transcriptome data and experimental identification show that solute carrier 35 member A2 (SLC35A2) is a prognostic marker of colorectal cancer; Authors - Yue Wang
human	IHC,WB	FEBS J; SLC35A2 expression drives breast cancer progression via ERK pathway activation; Authors - Xiaochen Yang
human	WB	Gene; Solute carrier family 35 member A2 regulates mitophagy through the PI3K/AKT/mTOR axis, promoting the proliferation, migration, and invasion of osteosarcoma cells; Authors - Xiaohui Luo