SLC37A1 Polyklonaler Antikörper

SLC37A1 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24582-1-AP

Synonyme

G 3 P permease, G 3 P transporter, G3PP, Glycerol 3 phosphate permease, SLC37A1, SPX1

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Galerie der Validierungsdaten

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  WB analysis using 24582-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24582-1-AP (SLC37A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 24582-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 24582-1-AP (SLC37A1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 24582-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using SLC37A1 antibody (24582-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, MCF-7-Zellen, Rattennierengewebe
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

24582-1-AP bindet in WB, IF, ELISA SLC37A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC37A1 fusion protein Ag20180
• Vollständiger Name: solute carrier family 37 (glycerol-3-phosphate transporter), member 1
• Berechnetes Molekulargewicht: 533 aa, 58 kDa
• Beobachtetes Molekulargewicht: 60-66 kDa
• GenBank-Zugangsnummer: BC152997
• Gene symbol: SLC37A1
• Gene ID (NCBI): 54020
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC37A1 (also known as SPX1) is one of the SLC37 family members, localized in the endoplasmic reticulum (ER) membrane (PMID:29719821). The SLC37A1 protein also shares 30% sequence identity to GlpT, suggesting that SLC37A1 could be a mammalian glycerol-3-phosphate transporter. The SLC37A1 gene is widely expressed, with the highest transcript levels in adult kidney, bone marrow, intestine, spleen, and liver (PMID:24745989). In addition, the SLC37A1 protein shares 59% homology to SLC37A2, 35% homology to SLC37A3, and 22% homology to SLC37A4 (PMID:23506893). SLC37A1 is a 533 amino-acid protein with a calculated molecular weight of 58 kDa and observed molecular weight 60-66kDa.