SLC37A2 Polyklonaler Antikörper

SLC37A2 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 20469-1-AP

Synonyme

pp11662, SLC37A2, SPX2, Sugar phosphate exchanger 2

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  WB analysis of mouse thymus using 20469-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 20469-1-AP (SLC37A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20469-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20469-1-AP (SLC37A2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 20469-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 20469-1-AP (SLC37A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 20469-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 20469-1-AP (SLC37A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Thymusgewebe, HeLa-Zellen, Mausmilzgewebe, Ratten-Thymusgewebe, RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:300-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

20469-1-AP bindet in WB, IHC, ELISA SLC37A2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC37A2 fusion protein Ag14306
• Vollständiger Name: solute carrier family 37 (glycerol-3-phosphate transporter), member 2
• Berechnetes Molekulargewicht: 505 aa, 55 kDa
• Beobachtetes Molekulargewicht: 50-75 kDa
• GenBank-Zugangsnummer: BC051314
• Gene symbol: SLC37A2
• Gene ID (NCBI): 219855
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The solute carrier family 37 (SLC37) consists of four sugar-phosphate exchangers, A1, A2, A3, and A4, which are anchored in the endoplasmic reticulum (ER) membrane (PMID:23506893). Solute Carrier Family 37 Member 2 (SLC37A2), also knows as SPX2, was firstly identified in a work, conducted on mice and aimed to detect cAMP inducible genes playing a role in promoting cholesterol efflux from the macrophage cell line RAW264 via apoE and apoA1 (PMID:11004510). Interestingly, of the four SLC37A members, SLC37A2 displays the highest level of transcript abundance in neutrophils and macrophages ,indicating that SLC37A2 may play an essential role in regulating innate immune function (PMID:32428862).

Publikationen

Species	Application	Title
human	WB,IHC	Cell Biol Toxicol; hucMSC-Ex alleviates inflammatory bowel disease in mice by enhancing M2-type macrophage polarization via the METTL3-Slc37a2-YTHDF1 axis; Authors - Xinwei Xu
mouse	WB	J Asian Nat Prod Res; Chlorogenic acid targets SLC37A2 to inhibit macrophage activation via ER-dependent NF-κB and NLRP3 signaling pathways against sepsis-induced acute lung injury; Authors - Yang Chen