SLC37A4 Polyklonaler Antikörper

SLC37A4 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 20612-1-AP

Synonyme

Glucose-6-phosphate exchanger SLC37A4, Glucose-5-phosphate transporter, G6PT3, G6PT2, G6PT1

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  WB analysis of mouse liver using 20612-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20612-1-AP (SLC37A4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 20612-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20612-1-AP (SLC37A4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 20612-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20612-1-AP (SLC37A4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20612-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20612-1-AP (SLC37A4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 20612-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20612-1-AP (SLC37A4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 20612-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20612-1-AP (SLC37A4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 20612-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20612-1-AP (SLC37A4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 20612-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 20612-1-AP (SLC37A4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 20612-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 20612-1-AP (SLC37A4 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 20612-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 20612-1-AP (SLC37A4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 20612-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 20612-1-AP (SLC37A4 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen, Mausnierengewebe, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

20612-1-AP bindet in WB, IHC, IF, ELISA SLC37A4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC37A4 fusion protein Ag14660
• Vollständiger Name: solute carrier family 37 (glucose-6-phosphate transporter), member 4
• Berechnetes Molekulargewicht: 429 aa, 46 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC015650
• Gene symbol: SLC37A4
• Gene ID (NCBI): 2542
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cell Death Discov; Circ_0000235 targets MCT4 to promote glycolysis and progression of bladder cancer by sponging miR-330-5p; Authors - Jianye Zhong
human	IF	Sci Rep; SLC37A4, gene responsible for glycogen storage disease type 1b, regulates gingival epithelial barrier function via JAM1 expression; Authors - Keita Tanigaki
human	WB	J Adv Res; SRSF9 mediates oncogenic RNA splicing of SLC37A4 via liquid-liquid phase separation to promote oral cancer progression; Authors - Qiu Peng