SLC38A3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SLC38A3 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

17

Kat-Nr. : 14315-1-AP

Synonyme

G17, NAT1, SLC38A3, SN1, SNAT3

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Galerie der Validierungsdaten

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  WB analysis using 14315-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14315-1-AP (SLC38A3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 14315-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14315-1-AP (SLC38A3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 14315-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14315-1-AP (SLC38A3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 14315-1-AP

  IP Result of anti-SLC38A3 (IP:14315-1-AP, 3ug; Detection:14315-1-AP 1:1000) with mouse heart tissue lysate 4000ug.

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  IHC staining of human lung cancer using 14315-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 14315-1-AP (SLC38A3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung cancer using 14315-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 14315-1-AP (SLC38A3 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HEK-293 using 14315-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 14315-1-AP (SLC38A3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 14315-1-AP

  1X10^6 HeLa cells were stained with 0.2ug SLC38A3 antibody (14315-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Mauslebergewebe, Maus-Pankreasgewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 15 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

14315-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA SLC38A3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC38A3 fusion protein Ag5553
• Vollständiger Name: solute carrier family 38, member 3
• Berechnetes Molekulargewicht: 504 aa, 56 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC042875
• Gene symbol: SLC38A3
• Gene ID (NCBI): 10991
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC38A3, also known as SNAT3 or SN1, belongs to the SLC38 family of sodium-coupled neutral amino acid transporters. SLC38A3 mediates glutamine transport with sodium and proton coupled manner, and it contributes to ammonia metabolism in the liver and brain. SLC38A3 is highly expressed in the liver and brain astrocytes, with modest expression in the kidney, heart, and muscle. SLC38A3 may migrate higher than the predicted 56 kDa due to the glycosylation.

Publikationen

Species	Application	Title
mouse	WB	Environ Pollut; Long-term exposure changes the environmentally relevant bis(2-ethylhexyl) phthalate to be a neuro-hazardous substance disrupting neural homeostasis in emotional and cognitive functions; Authors - Jae Soon Kang
mouse	WB,IF,CoIP	Int J Mol Sci; The Role of Nrf2 Transcription Factor and Sp1-Nrf2 Protein Complex in Glutamine Transporter SN1 Regulation in Mouse Cortical Astrocytes Exposed to Ammonia.; Authors - Katarzyna Dąbrowska
mouse	IHC	Cancer Lett; Amino acid transporter SLC38A3 promotes metastasis of non-small cell lung cancer cells by activating PDK1.; Authors - Yanhui Wang
human	WB	Cancer Lett; RCCD1 depletion attenuates TGF-β-induced EMT and cell migration by stabilizing cytoskeletal microtubules in NSCLC cells.; Authors - Junyu Wu
mouse	IF	iScience; Increased NMDARs in neurons and glutamine synthetase in astrocytes underlying autistic-like behaviors of Gabrb1-/- mice; Authors - Jing Wang
mouse	WB	Int J Mol Sci; Sex Modulates Response to Renal-Tubule-Targeted Insulin Receptor Deletion in Mice; Authors - Soha Sohail