SLC38A4 Polyklonaler Antikörper

SLC38A4 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Rezensionen (1)

Kat-Nr. : 20857-1-AP

Synonyme

Amino acid transporter A3, ATA3, NAT3, PAAT, SLC38A4, SNAT4

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  WB analysis using 20857-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20857-1-AP (SLC38A4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 20857-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20857-1-AP (SLC38A4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 20857-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20857-1-AP (SLC38A4 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 20857-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20857-1-AP (SLC38A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 20857-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20857-1-AP (SLC38A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 20857-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20857-1-AP (SLC38A4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 20857-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20857-1-AP (SLC38A4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 20857-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20857-1-AP (SLC38A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 20857-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20857-1-AP (SLC38A4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Jurkat-Zellen, L02-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Leberkarzinomgewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 2 publications below

Produktinformation

20857-1-AP bindet in WB, IHC, IF, ELISA SLC38A4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC38A4 fusion protein Ag14827
• Vollständiger Name: solute carrier family 38, member 4
• Berechnetes Molekulargewicht: 547 aa, 61 kDa
• Beobachtetes Molekulargewicht: 61 kDa
• GenBank-Zugangsnummer: BC101827
• Gene symbol: SLC38A4
• Gene ID (NCBI): 55089
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Clin Transl Med; Melatonin inhibits lipid accumulation to repress prostate cancer progression by mediating the epigenetic modification of CES1.; Authors - Lijie Zhou
human	WB,IF	Pathol Res Pract; Assisted reproductive technology causes reduced expression of amino acid transporters in human full-term placentas; Authors - Qingge Jia
pig	WB,IF	J Clin Invest; Arteriovenous metabolomics in pigs reveals CFTR regulation ofmetabolism inmultiple organs; Authors - Hosung Bae

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Christopher (Verified Customer) (06-13-2024)

EXCELLENT ANTIBODY! I had very clean bands using a 1:1000 dilution with the recommended blocking buffers.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Placenta tissue