SLC38A7 Rekombinanter Antikörper

SLC38A7 Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240147C3

Kat-Nr. : 83346-6-RR

Synonyme

SNAT7, Solute carrier family 38 member 7, Sodium-coupled neutral amino acid transporter 7, FLJ12724, FLJ10815

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  WB analysis of Caco-2 using 83346-6-RR

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 83346-6-RR (SLC38A7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 83346-6-RR

  human liver tissue were subjected to SDS PAGE followed by western blot with 83346-6-RR (SLC38A7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  FC experiment of Caco-2 using 83346-6-RR

  1x10^6 Caco-2 cells were intracellularly stained with 0.25 ug SLC38A7 Recombinant antibody (83346-6-RR, Clone:240147C3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of U-251 using 83346-6-RR

  1x10^6 U-251 cells were intracellularly stained with 0.25 ug SLC38A7 Recombinant antibody (83346-6-RR, Clone:240147C3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83346-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83346-6-RR against Human SLC38A7 were performed. The affinity constant is 1.31 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Caco-2-Zellen, humanes Lebergewebe
• Erfolgreiche Detektion in FC (Intra): Caco-2-Zellen, U-251-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83346-6-RR bindet in WB, FC (Intra), ELISA SLC38A7 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SLC38A7 fusion protein Ag35081
• Vollständiger Name: solute carrier family 38, member 7
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC001961
• Gene symbol: SLC38A7
• Gene ID (NCBI): 55238
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC38A7 also known as SNAT7, is a member of the SLC38 family that encodes sodium-coupled neutral amino acid transporters. SLC38A7 is a system N transporter that has a substrate preference for L-glutamine. It also transports other amino acids with polar side chains, as well as L-histidine and L-alanine (PMID: 21511949).