SLC5A8 Polyklonaler Antikörper

SLC5A8 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(14)

Kat-Nr. : 21433-1-AP

Synonyme

AIT, Apical iodide transporter, SMCT, SMCT1, Sodium iodide-related cotransporter

---

• 

  WB analysis using 21433-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21433-1-AP (SLC5A8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 21433-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21433-1-AP (SLC5A8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse kidney using 21433-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21433-1-AP (SLC5A8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 21433-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21433-1-AP (SLC5A8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 21433-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 21433-1-AP (SLC5A8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 21433-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 21433-1-AP (SLC5A8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse kidney using 21433-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using SLC5A8 antibody (21433-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse kidney using 21433-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using SLC5A8 antibody (21433-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, humanes Hirngewebe, Maushirngewebe, Mausnierengewebe, Rattennierengewebe
• Erfolgreiche Detektion in IHC: Rattennierengewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mausnierengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:5000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 10 publications below
• IHC: See 6 publications below
• IF: See 4 publications below

Produktinformation

21433-1-AP bindet in WB, IHC, IF-P, ELISA SLC5A8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC5A8 fusion protein Ag16041
• Vollständiger Name: solute carrier family 5 (iodide transporter), member 8
• Berechnetes Molekulargewicht: 610 aa, 67 kDa
• Beobachtetes Molekulargewicht: 67 kDa
• GenBank-Zugangsnummer: BC110492
• Gene symbol: SLC5A8
• Gene ID (NCBI): 160728
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The sodium-coupled monocarboxylate transporter 1 (SMCT1; SLC5A8), belonging to the SLC5A family, is a 67-kDa protein that functions as a Na+-coupled electrogenic transporter for SCFAs. SLC5A mRNA and protein are expressed in healthy human, rat, and mouse colon. The protein is localized to the apical membrane of the colonocytes. Immunohistochemical studies revealed the selective localization of SMCT1 on the brush border in the villi of the terminal ileum and crypts of the colon in mice.

Publikationen

Species	Application	Title
human	IHC,IF	Cell Metab; The probiotic L. casei Zhang slows the progression of acute and chronic kidney disease.; Authors - Han Zhu
rat,human	WB	J Ethnopharmacol; Microbiota-derived short-chain fatty acids mediate the effects of dengzhan shengmai in ameliorating cerebral ischemia via the gut-brain axis; Authors - Hui-Hui Guo
mouse	WB,IHC	Chem Biol Interact; Downregulation of OATP2B1 by proinflammatory cytokines leads to 5-ASA hyposensitivity in Ulcerative colitis; Authors - Ping Li
human	WB	Exp Mol Med; Impaired Na+-K+-ATPase signaling in renal proximal tubule contributes to hyperuricemia-induced renal tubular injury.; Authors - Jing Xiao
human	WB	Front Pharmacol; Ramulus Mori (Sangzhi) Alkaloids (SZ-A) Ameliorate Glucose Metabolism Accompanied by the Modulation of Gut Microbiota and Ileal Inflammatory Damage in Type 2 Diabetic KKAy Mice.; Authors - Quan Liu
mouse	IHC	Sci Rep; Gut-derived butyrate suppresses ocular surface inflammation.; Authors - Laura Schaefer