SLC6A19 Polyklonaler Antikörper

SLC6A19 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 29372-1-AP

Synonyme

Sodium-dependent neutral amino acid transporter B(0)AT1, HND, B0AT1

---

• 

  WB analysis using 29372-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29372-1-AP (SLC6A19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 29372-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29372-1-AP (SLC6A19 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 29372-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29372-1-AP (SLC6A19 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 29372-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29372-1-AP (SLC6A19 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 29372-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 29372-1-AP (SLC6A19 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human small intestine using 29372-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 29372-1-AP (SLC6A19 antibody) at dilution of 1:200 (under 10x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

29372-1-AP bindet in WB, IHC, ELISA SLC6A19 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC6A19 fusion protein Ag31076
• Vollständiger Name: solute carrier family 6 (neutral amino acid transporter), member 19
• Berechnetes Molekulargewicht: 634 aa, 71 kDa
• Beobachtetes Molekulargewicht: 71 kDa
• GenBank-Zugangsnummer: BC148801
• Gene symbol: SLC6A19
• Gene ID (NCBI): 340024
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC6A19, also named as B0AT1, encodes system B(0) transmembrane protein that mediates epithelial resorption of neutral amino acids across the apical membrane in kidney and intestine (PubMed: 15044460). The protein contains 634 amino acids and has 12 predicted transmembrane regions with highly expression in human kidney and small intestine and minimal in pancreas (PubMed: 15286787). The expression of SLC6A19 in intestinal cells depends on angiotensin converting enzyme 2 which is the cellular receptor for SARS-CoV-2 (PubMed: 32132184). Mutations in SLC6A19 cause Hartnup disease (PMID: 15286787, PMID: 15286788).

Publikationen

Species	Application	Title
rat	WB	Am J Pathol; Role of Kir5.1 (Kcnj16) Channels in Regulating Renal Ammonia Metabolism during Metabolic Acidosis in Dahl Salt-Sensitive Rats; Authors - Biyang Xu
mouse	IHC	J Ethnopharmacol; Metabolomics and molecular docking-directed anti-obesity study of the ethanol extract from Gynostemma pentaphyllum (Thunb.) Makino; Authors - Suyun Xu