Dopamine Transporter/DAT Polyklonaler Antikörper

Dopamine Transporter/DAT Polyklonal Antikörper für WB, IHC, IF-P, IF-Fro, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF-P, IF-Fro, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(57)

Rezensionen (1)

Kat-Nr. : 22524-1-AP

Synonyme

2, SLC6A3, DA transporter, DAT, DAT1

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  WB analysis using 22524-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22524-1-AP (DAT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 22524-1-AP

  IP result of anti-Dopamine Transporter/DAT (IP:22524-1-AP, 4ug; Detection:22524-1-AP 1:1000) with mouse brain tissue lysate 1720 ug.

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  IHC staining of mouse brain using 22524-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22524-1-AP (DAT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 22524-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22524-1-AP (DAT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 22524-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22524-1-AP (DAT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 22524-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DAT antibody (22524-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 22524-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using Dopamine Transporter/DAT antibody (22524-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe
• Erfolgreiche Detektion in IF-Fro: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 45 publications below
• IHC: See 6 publications below
• IF: See 13 publications below

Produktinformation

22524-1-AP bindet in WB, IHC, IF-P, IF-Fro, IP, ELISA Dopamine Transporter/DAT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Dopamine Transporter/DAT fusion protein Ag18297
• Vollständiger Name: solute carrier family 6 (neurotransmitter transporter, dopamine), member 3
• Berechnetes Molekulargewicht: 620 aa, 68 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC133003
• Gene symbol: DAT
• Gene ID (NCBI): 6531
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DAT, also name as SLC6A3, is dopamine transporter which is a member of the sodium- and chloride- dependent neurotransmitter transporter family. Dopamine(DA) released from neurons is cleared by the DA transporter (DAT). Altered dopaminergic signaling is linked to multiple neuropsychiatric disorders, such as attention deficit hyperactive disorder, mood disorders, schizophrenia, autism and so on (PMID:30755521). 22524-1-AP can detect 70~80kDa band, which is consistent with the report (PMID:12746456).

Publikationen

Species	Application	Title
mouse	IF	Nature; Reversing a model of Parkinson's disease with in situ converted nigral neurons.; Authors - Hao Qian
mouse		Sci Adv; Phosphoglycerate kinase is a central leverage point in Parkinson's disease-driven neuronal metabolic deficits; Authors - Alexandros C Kokotos
mouse	WB	Mov Disord; Fine Particulate Matter Triggers α-Synuclein Fibrillization and Parkinson-like Neurodegeneration; Authors - Xin Yuan
rat	WB	Phytomedicine; Gastrodin relieves Parkinson's disease-related motor deficits by facilitating the MEK-dependent VMAT2 to maintain dopamine homeostasis; Authors - Meihuan Zhao
rat	WB	J Affect Disord; Dysregulation of striatal dopamine D2/D3 receptor-mediated by hypocretin induces depressive behaviors in rats; Authors - Bing Chen
human	WB	Br J Pharmacol; Andrographolide alleviates parkinsonism in MPTP-PD mice via targeting DRP1-mediated mitochondrial fission.; Authors - Ji Geng

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Angie (Verified Customer) (01-20-2025)

Overnight incubation of primary antibody (used at 1:200) at 4 °C followed by 1 h incubation of secondary antibody (Alexa Fluor Donkey anti-rabbit 488, 1:500) at room temperature.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Differentiated SHSY-5Y

Dopamine Transporter/DAT Antibody Immunofluorescence validation (1:200 dilution) in Differentiated SHSY-5Y (Cat no:22524-1-AP)