SLC7A11/xCT Rekombinanter Antikörper

SLC7A11/xCT Rekombinant Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230227C5

Kat-Nr. : CL488-82115-2

Synonyme

SLC7A11, xCT, 230227C5, Amino acid transport system xc, Amino acid transport system xc-

---

• 

  IF Staining of HeLa using CL488-82115-2

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 SLC7A11/xCT antibody (CL488-82115-2, Clone: 230227C5 ) at dilution of 1:200.

• 

  FC experiment of HeLa using CL488-82115-2

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Coralite® Plus 488 Slc7a11/Xct Recombinant Antibody (CL488-82115-2, Clone:230227C5)(red), or 0.25 ug CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of A549 using CL488-82115-2

  1x10^6 A549 cells were intracellularly stained with 0.25 ug Coralite® Plus 488 Slc7a11/Xct Recombinant Antibody (CL488-82115-2, Clone:230227C5)(red), or 0.25 ug CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen, A549-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-82115-2 bindet in IF/ICC, FC (Intra) SLC7A11/xCT und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SLC7A11/xCT fusion protein Ag25431
• Vollständiger Name: solute carrier family 7, (cationic amino acid transporter, y+ system) member 11
• Berechnetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC012087
• Gene symbol: SLC7A11
• Gene ID (NCBI): 23657
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC7A11 (also known as xCT) is a membrane transporter involved in the uptake of cystine. It promotes glutathione synthesis through the uptake of cystine and the concomitant release of glutamate. This, in turn, protects cells from oxidative stress, maintains cellular redox balance, and prevents cell death induced by lipid peroxidation. SLC7A11 has been identified as a potential target for cancer therapeutics. It is overexpressed in multiple malignant tumors and is closely associated with the growth, prognosis, metastasis, and treatment response of various cancers including lung cancer.