SLITRK6 Polyklonaler Antikörper

SLITRK6 Polyklonal Antikörper für FC, IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, FC,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21150-1-AP

Synonyme

SLIT and NTRK like protein 6, SLITRK6

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Galerie der Validierungsdaten

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  WB analysis of A549 using 21150-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21150-1-AP (SLITRK6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 21150-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21150-1-AP (SLITRK6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 21150-1-AP

  Immunofluorescent analysis of HepG2 cells, using SLITRK6 antibody 21150-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  FC experiment of HepG2 using 21150-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug SLITRK6 antibody (21150-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Mauslungengewebe
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

21150-1-AP bindet in WB, IF, FC,ELISA SLITRK6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLITRK6 fusion protein Ag15701
• Vollständiger Name: SLIT and NTRK-like family, member 6
• Berechnetes Molekulargewicht: 841 aa, 95 kDa
• Beobachtetes Molekulargewicht: 95-100 kDa
• GenBank-Zugangsnummer: BC101070
• Gene symbol: SLITRK6
• Gene ID (NCBI): 84189
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLITRK6 is a member of the SLITRK family. Members of this family are integral membrane proteins that are characterized by two N-terminal leucine-rich repeat (LRR) domains and a C-terminal region that shares homology with trk neurotrophin receptors. This protein functions as a regulator of neurite outgrowth required for normal hearing and vision. Mutations in SLITRK6 gene are a cause of myopia and deafness.