SLN Polyklonaler Antikörper

SLN Polyklonal Antikörper für IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(13)

Kat-Nr. : 18395-1-AP

Synonyme

sarcolipin, SLN

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  IHC staining of human heart using 18395-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18395-1-AP (SLN Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 18395-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18395-1-AP (SLN Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of PC-3 using 18395-1-AP

  Immunofluorescent analysis of PC-3 cells using 18395-1-AP (SLN antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: PC-3-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 9 publications below
• IF: See 4 publications below

Produktinformation

18395-1-AP bindet in WB, IHC, IF/ICC, ELISA SLN und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Eichh?rnchen, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLN fusion protein Ag13176
• Vollständiger Name: sarcolipin
• Berechnetes Molekulargewicht: 31 aa, 4 kDa
• GenBank-Zugangsnummer: BC005261
• Gene symbol: SLN
• Gene ID (NCBI): 6588
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLN, also named as Sarcolipin, belongs to the sarcolipin family. It is associated with calcium ATPase SERCA1. It inhibits SERCA pumps. SLN, a key regulator of cardiac sarco(endo)plasmic reticulum (SR) Ca(2+) ATPase, is predominantly expressed in atria and mediates β-adrenergic responses. SLN can self-assembly to dimer and oligomer for playing importantphysiological function.

Publikationen

Species	Application	Title
mouse	WB	EBioMedicine; Maternal exercise intergenerationally drives muscle-based thermogenesis via activation of apelin-AMPK signaling.; Authors - Jun Seok Son
	WB	J Biol Chem; Identification of Small Ankyrin 1 as a Novel Sarco(endo)plasmic Reticulum Ca2+-ATPase 1 (SERCA1) Regulatory Protein in Skeletal Muscle.; Authors - Patrick F Desmond
mouse	WB	Skelet Muscle; Ca(2+) permeation and/or binding to CaV1.1 fine-tunes skeletal muscle Ca(2+) signaling to sustain muscle function.; Authors - Chang Seok Lee
		J Biol Chem; Interactions between small ankyrin 1 and sarcolipin coordinately regulate activity of the sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA1).; Authors - Patrick F Desmond
mouse	WB	J Endocrinol; ChREBP-β regulates thermogenesis in brown adipose tissue.; Authors - Chunchun Wei
mouse	IF	Exp Cell Res; Effect of sarcolipin-mediated cell transdifferentiation in sarcopenia-associated skeletal muscle fibrosis.; Authors - Qiu-Nan Chen