SMARCC1 Polyklonaler Antikörper

SMARCC1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 17722-1-AP

Synonyme

BAF155, BRG1 associated factor 155, CRACC1, Rsc8, SMARCC1, SRG3, SWI3

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Galerie der Validierungsdaten

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  WB analysis using 17722-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17722-1-AP (SMARCC1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 17722-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17722-1-AP (SMARCC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 17722-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17722-1-AP (SMARCC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 17722-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 17722-1-AP (SMARCC1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 17722-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17722-1-AP (SMARCC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 17722-1-AP

  IP Result of anti-SMARCC1 (IP:17722-1-AP, 4ug; Detection:17722-1-AP 1:600) with mouse brain tissue lysate 4000ug.

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  IHC staining of human kidney using 17722-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17722-1-AP (SMARCC1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 17722-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17722-1-AP (SMARCC1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 17722-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17722-1-AP (SMARCC1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 17722-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17722-1-AP (SMARCC1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 17722-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17722-1-AP (SMARCC1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 17722-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17722-1-AP (SMARCC1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 17722-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17722-1-AP (SMARCC1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 17722-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17722-1-AP (SMARCC1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 17722-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17722-1-AP (SMARCC1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 17722-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17722-1-AP (SMARCC1 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, Jurkat-Zellen, Rattenhirngewebe, Rattenhodengewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Eierstockgewebe, humanes Hirngewebe, humanes Hodengewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

17722-1-AP bindet in WB, IP, IHC, CoIP, ELISA SMARCC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SMARCC1 fusion protein Ag12109
• Vollständiger Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1
• Berechnetes Molekulargewicht: 1105 aa, 123 kDa
• Beobachtetes Molekulargewicht: 155-160 kDa
• GenBank-Zugangsnummer: BC050564
• Gene symbol: SMARCC1
• Gene ID (NCBI): 6599
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMARCC1, also named as SWI/SNF complex 155 kDa subunit or BAF155, is a 1105 amino acid protein, which belongs to the SMARCC family. SMARCC1 is expressed in brain, heart, muscle, placenta, lung, liver, muscle, kidney and pancreas. SMARCC1 is Involved in transcriptional activation and repression of select genes by chromatin remodeling. SMARCC1 may stimulate the ATPase activity of the catalytic subunit of the complex. SMARCC1 belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). The calculated molecular weight of TCEA1 is 123 kDa, but modified SMARCC1 is about 155-160 kDa. (PMID: 24025402)

Publikationen

Species	Application	Title
human	WB,IP	Cancer Discov; A role for SMARCB1 in synovial sarcomagenesis reveals that SS18-SSX induces canonical BAF destruction.; Authors - Jinxiu Li
mouse	CoIP	Nucleic Acids Res; HRP2-DPF3a-BAF complex coordinates histone modification and chromatin remodeling to regulate myogenic gene transcription.; Authors - Xu Zhu
		Mol Cell Biol; Growth Factor Independence 1B-Mediated Transcriptional Repression and Lineage Allocation Require Lysine-Specific Demethylase 1-Dependent Recruitment of the BHC Complex.; Authors - David McClellan