SMC4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SMC4 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 24758-1-AP

Synonyme

CAP C, CAPC, Chromosome-associated polypeptide C, hCAP C, hCAPC

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  WB analysis using 24758-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24758-1-AP (SMC4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 24758-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24758-1-AP (SMC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 24758-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24758-1-AP (SMC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 24758-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 24758-1-AP (SMC4 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 24758-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24758-1-AP (SMC4 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 24758-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24758-1-AP (SMC4 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 24758-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24758-1-AP (SMC4 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 24758-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24758-1-AP (SMC4 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 24758-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMC4 antibody (24758-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, C6-Zellen, HeLa-Zellen, MCF-7-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:200-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

24758-1-AP bindet in WB, IHC, IF/ICC, ELISA SMC4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SMC4 fusion protein Ag19156
• Vollständiger Name: structural maintenance of chromosomes 4
• Berechnetes Molekulargewicht: 1288 aa, 147 kDa
• Beobachtetes Molekulargewicht: 147-150 kDa
• GenBank-Zugangsnummer: BC106033
• Gene symbol: SMC4
• Gene ID (NCBI): 10051
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMC4, also named as Structural maintenance of chromosomes protein 4, is a 1288 amino acid protein, which is widely expressed and belongs to the SMC family, SMC4 subfamily. SMC4 is a Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The calculated molecular weight of SMC4 is 147 kDa, but modified SMC4 is about 160 kDa.

Publikationen

Species	Application	Title
human	WB	Hum Mol Genet; Single-cell transcription profiles in Bloom syndrome patients link BLM deficiency with altered condensin complex expression signatures.; Authors - Ipek Ilgin Gönenc
rat	IHC	Gene; Transcriptional profiling of rat skeletal muscle hypertrophy under restriction of blood flow.; Authors - Shouyu Xu
		Sci Rep; Cryo-Electron Tomography and Proteomics studies of centrosomes from differentiated quiescent thymocytes.; Authors - Johan Busselez
mouse,human	WB,IHC,IF	Cell Metab; The diapause-like colorectal cancer cells induced by SMC4 attenuation are characterized by low proliferation and chemotherapy insensitivity; Authors - Xuedan Sun ; KO Validated
human	WB,IHC	Int J Immunopathol Pharmacol; SMC4 serves as a potential marker for the diagnosis and prognosis of colon adenocarcinoma; Authors - Dawei Yang