SMG6 Monoklonaler Antikörper

SMG6 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

1D2G2

Kat-Nr. : 68109-1-Ig

Synonyme

C17orf31, EST1 like protein A, EST1A, Ever shorter telomeres 1A, hSmg5/7a, KIAA0732, SMG 6, Smg 6 homolog, SMG6, Telomerase subunit EST1A

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Galerie der Validierungsdaten

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  WB analysis using 68109-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68109-1-Ig (SMG6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

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  WB analysis of RAW 264.7 using 68109-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 68109-1-Ig (SMG6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, HEK-293-Zellen, HeLa-Zellen, MCF-7-Zellen, RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68109-1-Ig bindet in WB, ELISA SMG6 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SMG6 fusion protein Ag30182
• Vollständiger Name: Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans)
• Berechnetes Molekulargewicht: 160 kDa
• Beobachtetes Molekulargewicht: 160 kDa
• GenBank-Zugangsnummer: NM_017575
• Gene symbol: SMG6
• Gene ID (NCBI): 23293
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMG6, also known as C17orf31, EST1A, and hEST1A, is a component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini (PMID: 19179534). It promotes in vitro the ability of TERT to elongate telomeres (PMID: 12676087, 12699629). SMG6 also plays a role in the nonsense-mediated mRNA decay (NMD) pathway, providing the endonuclease activity near the premature translation termination codon that is needed to initiate NMD (PMID: 19060897, 20930030). Alternate splicing results in multiple transcript variants (PMID: 12699629, 14636577).