SMIM1 Polyklonaler Antikörper

SMIM1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 27849-1-AP

Synonyme

hypothetical LOC388588, LOC388588, Small integral membrane protein 1, SMIM1

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  WB analysis of HEK-293 using 27849-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 27849-1-AP (SMIM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 27849-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27849-1-AP (SMIM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 27849-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 27849-1-AP (SMIM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Mausnierengewebe, Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below

Produktinformation

27849-1-AP bindet in WB, ELISA SMIM1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SMIM1 fusion protein Ag27028
• Vollständiger Name: hypothetical LOC388588
• Beobachtetes Molekulargewicht: 9 kDa
• GenBank-Zugangsnummer: BC146957
• Gene symbol: SMIM1
• Gene ID (NCBI): 388588
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Small integral membrane protein 1(SMIM1) is a type II transmembrane phosphoprotein and displays the Vel blood group antigen at its carboxyl-terminus. SMIM1 is highly expressed in hematopoietic cells. It is associated with a variety of immune responses and plays an important role in RBC differentiation. SMIM1 can be used as a potential biomarker for the diagnosis of IDD (PMID: 30906890; 26452714).

Publikationen

Species	Application	Title
human	WB	Transfus Med Hemother; CRISPR/Cas9-Editing K562 Cell Line as a Potential Tool in Transfusion Applications: Knockout of Vel Antigen Gene; Authors - Jiaxuan Yang ; KO Validated