SMN Polyklonaler Antikörper

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• KD/KO Validated

SMN Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20451-1-AP

Synonyme

C BCD541, Component of gems 1, Gemin 1, SMN, SMN1, SMN1,SMN, SMN2, SMNC, SMNT, Survival motor neuron protein

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 20451-1-AP

  WB result of SMN antibody (20451-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMN transfected HeLa cells.

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  WB analysis of HEK-293 using 20451-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20451-1-AP (SMN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 20451-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20451-1-AP (SMN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 20451-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20451-1-AP (SMN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 20451-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20451-1-AP (SMN antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 20451-1-AP

  IP result of anti-SMN (IP:20451-1-AP, 4ug; Detection:20451-1-AP 1:300) with HEK-293 cells lysate 2000 ug.

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  IHC staining of human kidney using 20451-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20451-1-AP (SMN antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 20451-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20451-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human heart using 20451-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20451-1-AP (SMN antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 20451-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20451-1-AP (SMN antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 20451-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMN antibody (20451-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:200
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

20451-1-AP bindet in WB, IP, IHC, IF, ELISA SMN und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SMN fusion protein Ag14333
• Vollständiger Name: survival of motor neuron 2, centromeric
• Berechnetes Molekulargewicht: 282 aa, 30 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC000908
• Gene symbol: SMN2
• Gene ID (NCBI): 6607
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease characterized by loss of anterior horn cells in the spinal cord and concomitant symmetrical muscle weakness and atrophy (PMID: 16364894 ). SMA is caused by deletion or mutations of the survival motor neuron (SMN1) gene. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional (PMID: 17355180). A large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein (PMID: 10369862). The level of SMN protein correlates with phenotypic severity of SMA.

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