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  • Featured Product
  • KD/KO Validated

SMN (Human,Mouse,Rat) Monoklonaler Antikörper

SMN (Human,Mouse,Rat) Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

3C10B5

Kat-Nr. : 60154-2-Ig

Synonyme

C BCD541, Component of gems 1, Gemin 1, SMN, SMN (Human,Mouse,Rat), SMN1, SMN1,SMN, SMN2, SMNC, SMNT, Survival motor neuron protein

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of HeLa cells using SMN (Human,Mouse,Rat) Monoclonal antibody (60154-2-Ig)

    WB analysis of HeLa using 60154-2-Ig

    WB result of SMN (Human,Mouse,Rat) antibody (60154-2-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMN (Human,Mouse,Rat) transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using SMN (Human,Mouse,Rat) Monoclonal antibody (60154-2-Ig)

    WB analysis using 60154-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using SMN (Human,Mouse,Rat) Monoclonal antibody (60154-2-Ig)

    WB analysis of A375 using 60154-2-Ig

    A375 cells were subjected to SDS PAGE followed by western blot with 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using SMN (Human,Mouse,Rat) Monoclonal antibody (60154-2-Ig)

    IHC staining of mouse liver using 60154-2-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using SMN (Human,Mouse,Rat) Monoclonal antibody (60154-2-Ig)

    IHC staining of mouse liver using 60154-2-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SMN (Human,Mouse,Rat) Monoclonal antibody (60154-2-Ig)

    IHC staining of mouse brain using 60154-2-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SMN (Human,Mouse,Rat) Monoclonal antibody (60154-2-Ig)

    IHC staining of mouse brain using 60154-2-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60154-2-Ig (SMN (Human,Mouse,Rat) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SMN (Human,Mouse,Rat) Monoclonal antibody (60154-2-Ig)

    IF Staining of HepG2 using 60154-2-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMN (Human,Mouse,Rat) antibody (60154-2-Ig, Clone: 3C10B5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBHepG2-Zellen, A375-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, MOLT-4-Zellen, Raji-Zellen, T-47D-Zellen
Erfolgreiche Detektion in IHCMauslebergewebe, Maushirngewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IFHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunhistochemie (IHC)IHC : 1:500-1:2000
Immunfluoreszenz (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

60154-2-Ig bindet in WB, IHC, IF, ELISA SMN (Human,Mouse,Rat) und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen SMN (Human,Mouse,Rat) fusion protein Ag14333
Vollständiger Name survival of motor neuron 2, centromeric
Berechnetes Molekulargewicht 282 aa, 30 kDa
Beobachtetes Molekulargewicht 38 kDa
GenBank-ZugangsnummerBC000908
Gene symbol SMN2
Gene ID (NCBI) 6607
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The survival of motor neurons (SMN) genes are the disease genes of spinal muscular atrophy (SMA), a common motor neuron degenerative disease. The level of SMN protein correlates with phenotypic severity of SMA. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional, because a large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein. This antibody 60154-2-Ig can recognize human,mouse and rat SMN gene.

Protokolle

Produktspezifische Protokolle
WB protocol for SMN (Human,Mouse,Rat) antibody 60154-2-IgProtokoll herunterladen
IHC protocol for SMN (Human,Mouse,Rat) antibody 60154-2-IgProtokoll herunterladen
IF protocol for SMN (Human,Mouse,Rat) antibody 60154-2-IgProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen