SMNDC1 Polyklonaler Antikörper

SMNDC1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12178-1-AP

Synonyme

30 kDa splicing factor SMNrp, SMN related protein, SMNDC1, SMNR, SPF30

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Galerie der Validierungsdaten

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  WB analysis using 12178-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12178-1-AP (SMNDC1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 12178-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12178-1-AP (SMNDC1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human lung using 12178-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12178-1-AP (SMNDC1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 12178-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12178-1-AP (SMNDC1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 12178-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12178-1-AP (SMNDC1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 12178-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12178-1-AP (SMNDC1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 12178-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12178-1-AP (SMNDC1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human lymphoma using 12178-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12178-1-AP (SMNDC1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human lymphoma using 12178-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 12178-1-AP (SMNDC1 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 12178-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 12178-1-AP (SMNDC1 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, humanes Herzgewebe, humanes Lebergewebe, humanes Lungengewebe, Maus-Skelettmuskelgewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

12178-1-AP bindet in WB, IHC, IF, ELISA SMNDC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SMNDC1 fusion protein Ag2822
• Vollständiger Name: survival motor neuron domain containing 1
• Berechnetes Molekulargewicht: 238 aa, 27 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC011234
• Gene symbol: SMNDC1
• Gene ID (NCBI): 10285
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Survival motor neuron domain-containing protein 1 (SMNDC1), also known as survival of motor neuron-related-splicing factor 30 (SPF30) or SMN-related protein (SMNR), is a constituent of the spliceosome complex that plays an essential role in spliceosome assembly by recruiting U4/U5/U6 tri-snRNP to the pre-spliceosomal complex (PMID: 9817934; 11331595; 30904945). Both SMNDC1 and SMN1 contain a highly conserved central Tudor domain which functions as a protein-protein interaction surface and mediates binding to the spliceosomal Sm proteins (PMID: 11331595).