SMO Polyklonaler Antikörper

SMO Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

33

Rezensionen (1)

Kat-Nr. : 20787-1-AP

Synonyme

Gx, Protein Gx, SMO, SMOH, Smoothened homolog

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Galerie der Validierungsdaten

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  IHC staining of human prostate cancer using 20787-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20787-1-AP (SMO antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 20787-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20787-1-AP (SMO antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 20787-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20787-1-AP (SMO Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 20787-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20787-1-AP (SMO Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon cancer using 20787-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20787-1-AP (SMO antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 20787-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20787-1-AP (SMO antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 20787-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20787-1-AP (SMO antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 20787-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20787-1-AP (SMO antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 20787-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 20787-1-AP (SMO antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 20787-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 20787-1-AP (SMO antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Hodengewebe, humanes Kolonkarzinomgewebe, Maushodengewebe, Rattenhodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• IHC: See 4 publications below
• IF: See 6 publications below

Produktinformation

20787-1-AP bindet in IHC, IF, ELISA SMO und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Huhn, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SMO fusion protein Ag14322
• Vollständiger Name: smoothened homolog (Drosophila)
• Berechnetes Molekulargewicht: 787 aa, 86 kDa
• GenBank-Zugangsnummer: BC009989
• Gene symbol: SMO
• Gene ID (NCBI): 6608
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Smoothened (SMO) is a G protein-coupled receptor that is a component of the hedgehog (Hh) signaling pathway and is conserved from flies to humans. The Hh pathway plays central roles in animal development and stem-cell function. Defects in Hh signalling lead to birth defects and cancer in humans. Expressed on the primary cilium, SMO interacts with the patched protein, a receptor for Hh proteins.

Publikationen

Species	Application	Title
human,mouse		Mol Cancer; Circular RNA circIPO11 drives self-renewal of liver cancer initiating cells via Hedgehog signaling.; Authors - Yang Gu
chicken	IF	Sci Adv; Primary cilium remodeling mediates a cell signaling switch in differentiating neurons.; Authors - Gabriela Toro-Tapia
human	IHC	Nat Commun; TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling.; Authors - Rongxuan Zhu
mouse	IF	J Cell Biol; Sterol and oxysterol synthases near the ciliary base activate the Hedgehog pathway.; Authors - Sarah Findakly
human	IF	Acta Neuropathol Commun; Meningioma cells express primary cilia but do not transduce ciliary Hedgehog signals.; Authors - Sarah Findakly
human		Cell Death Discov; N6-methyladenine-mediated aberrant activation of the lncRNA SOX2OT-GLI1 loop promotes non-small-cell lung cancer stemness; Authors - Hongliang Dong

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (05-13-2019)

For IF, it could label the ciliary localisation of endogenous Smo; for WB, it could label many bands around 100 kd.

• Applications: Western Blot, Immunofluorescence,
• Cell Tissue Type: MEF