SMTNL2 Polyklonaler Antikörper

SMTNL2 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14782-1-AP

Synonyme

Smoothelin-like protein 2, Smoothelin like protein 2, smoothelin like 2

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  WB analysis of COLO 320 using 14782-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14782-1-AP (SMTNL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 14782-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 14782-1-AP (SMTNL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 14782-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 14782-1-AP (SMTNL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14782-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14782-1-AP (SMTNL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of COLO 320 using 14782-1-AP

  IP result of anti-SMTNL2 (IP:14782-1-AP, 4ug; Detection:14782-1-AP 1:500) with COLO 320 cells lysate 4000ug.

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  IF Staining of MCF-7 using 14782-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SMTNL2 antibody (14782-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, MCF-7-Zellen, Maus-Dünndarmgewebe, SGC-7901-Zellen
• Erfolgreiche IP: COLO 320-Zellen
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

14782-1-AP bindet in WB, IF/ICC, IP, ELISA SMTNL2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SMTNL2 fusion protein Ag6395
• Vollständiger Name: smoothelin-like 2
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 68-70 kDa
• GenBank-Zugangsnummer: BC112177
• Gene symbol: SMTNL2
• Gene ID (NCBI): 342527
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.