SNAP23 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SNAP23 Polyklonal Antikörper für FC, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

19

Kat-Nr. : 10825-1-AP

Synonyme

HsT17016, SNAP 23, SNAP23, SNAP23A, SNAP23B

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 10825-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10825-1-AP (SNAP23 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10825-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10825-1-AP (SNAP23 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10825-1-AP

  IP Result of anti-SNAP23 (IP:10825-1-AP, 4ug; Detection:10825-1-AP 1:500) with HeLa cells lysate 880ug.

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  IHC staining of human prostate cancer using 10825-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10825-1-AP (SNAP23 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 10825-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10825-1-AP (SNAP23 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 10825-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10825-1-AP (SNAP23 Antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of HepG2 using 10825-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human SNAP23 (10825-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:200
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 16 publications below
• IHC: See 3 publications below
• IF: See 7 publications below

Produktinformation

10825-1-AP bindet in WB, IP, IHC, IF, FC, ELISA SNAP23 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SNAP23 fusion protein Ag1269
• Vollständiger Name: synaptosomal-associated protein, 23kDa
• Berechnetes Molekulargewicht: 23 kDa
• Beobachtetes Molekulargewicht: 23-25 kDa
• GenBank-Zugangsnummer: BC000148
• Gene symbol: SNAP23
• Gene ID (NCBI): 8773
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IF	J Extracell Vesicles; Identification of the SNARE complex that mediates the fusion of multivesicular bodies with the plasma membrane in exosome secretion; Authors - Chuqi Liu ; KO Validated
human	WB,IF	Nat Commun; KRAS regulation by small non-coding RNAs and SNARE proteins.; Authors - Yonglu Che
rat	IF	Aging Cell; Knockdown of astrocytic Grin2a aggravates β-amyloid-induced memory and cognitive deficits through regulating nerve growth factor.; Authors - Zunshu Du
mouse	IF	Cell Death Differ; Transglutaminase 2 crosslinks zona pellucida glycoprotein 3 to prevent polyspermy.; Authors - Zhaokang Cui
human	WB	Oncogene; Small extracellular vesicles containing miR-30a-3p attenuate the migration and invasion of hepatocellular carcinoma by targeting SNAP23 gene.; Authors - Chengdong Liu ; KD Validated
human	WB	Int J Mol Sci; Exogenous H2S Attenuates Hypertension by Regulating Renin Exocytosis under Hyperglycaemic and Hyperlipidaemic Conditions; Authors - Ning Liu

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