SNAPIN Polyklonaler Antikörper

SNAPIN Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 10055-1-AP

Synonyme

SNAP associated protein, SNAP25BP, SNAPAP, SNAPIN

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Galerie der Validierungsdaten

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  WB analysis of A375 using 10055-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 10055-1-AP (SNAPIN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10055-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10055-1-AP (SNAPIN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 10055-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10055-1-AP (SNAPIN Antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10055-1-AP

  IP Result of anti-SNAPIN (IP:10055-1-AP, 5ug; Detection:10055-1-AP 1:300) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human testis using 10055-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10055-1-AP (SNAPIN Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 10055-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10055-1-AP (SNAPIN Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human brain using 10055-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10055-1-AP (SNAPIN Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 10055-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10055-1-AP (SNAPIN Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 10055-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SNAPIN antibody (10055-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 10055-1-AP

  Immunofluorescent analysis of HepG2 cells using 10055-1-AP (SNAPIN antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 10055-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug SNAPIN antibody (10055-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, HEK-293-Zellen, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

10055-1-AP bindet in WB, IP, IHC, IF, FC, ELISA SNAPIN und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SNAPIN fusion protein Ag0101
• Vollständiger Name: SNAP-associated protein
• Berechnetes Molekulargewicht: 15 kDa
• Beobachtetes Molekulargewicht: 15-18 kDa
• GenBank-Zugangsnummer: BC000761
• Gene symbol: SNAPIN
• Gene ID (NCBI): 23557
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Snapin, a protein of relative molecular weight of 15kd, is implicated in neurotransmission by binding to SNAP-25. Snapin was enriched in neurons and exclusively located on synaptic vesicle membrane, which may be a PKA target for modulating transmitter release through the cAMP-dependent signal-transduction pathway. This anitbody can recognize the 15-18kd(Monomer) and 30-36kd(Dimer) forms of SNAPIN.

Publikationen

Species	Application	Title
human		J Thromb Haemost; Dysregulation of PLDN (pallidin) is a mechanism for platelet dense granule deficiency in RUNX1 haplodeficiency.; Authors - G F Mao
human	WB	Am J Hum Genet; A BLOC-1 mutation screen reveals that PLDN is mutated in Hermansky-Pudlak Syndrome type 9.; Authors - Cullinane Andrew R AR
mouse	WB	Autophagy; Carbon nanotubes, but not spherical nanoparticles, block autophagy by a shape-related targeting of lysosomes in murine macrophages.; Authors - Vanessa Cohignac
human	WB	Autophagy; HIV-1 Vpr protein impairs lysosome clearance causing SNCA/alpha-synuclein accumulation in neurons.; Authors - Maryline Santerre
human	WB,IF	Autophagy; SNAPIN is critical for lysosomal acidification and autophagosome maturation in macrophages.; Authors - Bo Shi
human	WB	J Cell Biol; LAMTOR/Ragulator is a negative regulator of Arl8b- and BORC-dependent late endosomal positioning.; Authors - Przemyslaw A Filipek

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