SNCB Polyklonaler Antikörper

SNCB Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10498-1-AP

Synonyme

Beta synuclein, SNCB, synuclein, beta

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 10498-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10498-1-AP (SNCB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10498-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10498-1-AP (SNCB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10498-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10498-1-AP (SNCB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 10498-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10498-1-AP (SNCB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10498-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10498-1-AP (SNCB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10498-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10498-1-AP (SNCB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10498-1-AP

  IP Result of anti-SNCB (IP:10498-1-AP, 4ug; Detection:10498-1-AP 1:500) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human breast cancer using 10498-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10498-1-AP (SNCB antibody) at dilution of 1:10 (under 10x lens).

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  IF Staining of HepG2 using 10498-1-AP

  Immunofluorescent analysis of HepG2 cells, using SNCB antibody 10498-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HepG2 using 10498-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug SNCB antibody (10498-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:10-1:100
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

10498-1-AP bindet in WB, IP, IHC, IF, FC, ELISA SNCB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SNCB fusion protein Ag0772
• Vollständiger Name: synuclein, beta
• Berechnetes Molekulargewicht: 14 kDa
• Beobachtetes Molekulargewicht: 14-18 kDa
• GenBank-Zugangsnummer: BC002902
• Gene symbol: SNCB
• Gene ID (NCBI): 6620
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.