SNRPD2 Polyklonaler Antikörper

SNRPD2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 14789-1-AP

Synonyme

Sm D2, SMD2, snRNP core protein D2, SNRPD2

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  WB analysis of A2780 using 14789-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 14789-1-AP (SNRPD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A2780 using 14789-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 14789-1-AP (SNRPD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 14789-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 14789-1-AP (SNRPD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 14789-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 14789-1-AP (SNRPD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 14789-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14789-1-AP (SNRPD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 14789-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14789-1-AP (SNRPD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin using 14789-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 14789-1-AP (SNRPD2 Antibody) at dilution of 1:50.

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  IHC staining of human lung using 14789-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 14789-1-AP (SNRPD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 14789-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 14789-1-AP (SNRPD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 14789-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14789-1-AP (SNRPD2 Antibody) at dilution of 1:50.

• 

  IHC staining of human lung cancer using 14789-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14789-1-AP (SNRPD2 Antibody) at dilution of 1:50.

• 

  IHC staining of human skin using 14789-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 14789-1-AP (SNRPD2 Antibody) at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A2780-Zellen, A549-Zellen, HL-60-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautgewebe, humanes Lungenkarzinomgewebe, humanes Lungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

14789-1-AP bindet in WB, IHC, ELISA SNRPD2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SNRPD2 fusion protein Ag6443
• Vollständiger Name: small nuclear ribonucleoprotein D2 polypeptide 16.5kDa
• Berechnetes Molekulargewicht: 14 kDa
• Beobachtetes Molekulargewicht: 14-16 kDa
• GenBank-Zugangsnummer: BC000486
• Gene symbol: SNRPD2
• Gene ID (NCBI): 6633
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Systemic lupus erythematosus is characterized by antibodies to a variety of intracellular self-antigens, such as dsDNA and Sm, and these serve as hallmarks in the diagnosis of systemic autoimmune diseases. SNRPD2 is one of Sm protein, and is required for pre-mRNA splicing, snRNP biogenesis.

Publikationen

Species	Application	Title
human	WB	RNA Biol; Nuclear retention element recruits U1 snRNP components to restrain spliced lncRNAs in the nucleus.; Authors - Sikandar Azam