SOAT2 Polyklonaler Antikörper

SOAT2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 21852-1-AP

Synonyme

EC:2.3.1.26, ARGP2, Acyl-coenzyme A:cholesterol acyltransferase 2, ACAT-2, ACAT2

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  WB analysis using 21852-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21852-1-AP (SOAT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 21852-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21852-1-AP (SOAT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 21852-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21852-1-AP (SOAT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 21852-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21852-1-AP (SOAT2 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human small intestine using 21852-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21852-1-AP (SOAT2 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 21852-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21852-1-AP (SOAT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 21852-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SOAT2 antibody (21852-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 21852-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SOAT2 antibody (21852-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, L02-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 2 publications below

Produktinformation

21852-1-AP bindet in WB, IHC, IF/ICC, ELISA SOAT2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SOAT2 fusion protein Ag16364
• Vollständiger Name: sterol O-acyltransferase 2
• Berechnetes Molekulargewicht: 522 aa, 60 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC096090
• Gene symbol: SOAT2
• Gene ID (NCBI): 8435
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SOAT2, also named as ACAT2, belongs to the membrane-bound acyltransferase family and Sterol o-acyltransferase subfamily. SOAT2 is expressed preferentially in enterocytes of the intestine and hepatocytes of the liver. The role of SOAT2 in generating esterified cholesterol (EC) that is incorporated directly into atherogenic lipoproteins has made it an attractive target in dyslipidemia management and prevention. (PMID: 29246491)

Publikationen

Species	Application	Title
human	IHC	Cell Death Discov; Cholesterol activates the Wnt/PCP-YAP signaling in SOAT1-targeted treatment of colon cancer.; Authors - Huanji Xu
mouse	IHC	Nat Commun; Increased SOAT2 expression in aged regulatory T cells is associated with altered cholesterol metabolism and reduced anti-tumor immunity; Authors - Mingjiong Zhang
human	WB	J Lipid Res; Impaired ApoB Secretion Triggers Enhanced Secretion of ApoE to Maintain Triglyceride Homeostasis in Hepatoma Cells; Authors - Kotomi Shinozaki ; KO Validated