SOD2 Polyklonaler Antikörper

SOD2 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (4)

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

280

Rezensionen (1)

Kat-Nr. : 24127-1-AP

Synonyme

IPO B, Mn SOD, MNSOD, SOD2, SODM

---

Galerie der Validierungsdaten

• 

  WB analysis using 24127-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24127-1-AP (SOD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 24127-1-AP

  IP result of anti-SOD2(IP:24127-1-AP, 4ug; Detection:24127-1-AP 1:10000) with mouse brain tissue lysate 1280 ug.

• 

  IHC staining of human liver cancer using 24127-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24127-1-AP (SOD2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 24127-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24127-1-AP (SOD2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 24127-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24127-1-AP (SOD2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 24127-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24127-1-AP (SOD2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 24127-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24127-1-AP (SOD2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 24127-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24127-1-AP (SOD2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HUVEC using 24127-1-AP

  Immunofluorescent analysis of HUVEC cells using 24127-1-AP (SOD2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

• 

  IF Staining of HUVEC using 24127-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using SOD2 antibody (24127-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HUVEC using 24127-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using SOD2 antibody (24127-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: DU 145-Zellen, HEK-293-Zellen, HeLa-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HUVEC-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 257 publications below
• IHC: See 25 publications below
• IF: See 20 publications below
• IP: See 4 publications below
• ELISA: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

24127-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA SOD2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Huhn, Maus, Ratte, Rind, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SOD2 fusion protein Ag21388
• Vollständiger Name: superoxide dismutase 2, mitochondrial
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC016934
• Gene symbol: SOD2
• Gene ID (NCBI): 6648
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Superoxide dismutase 2 (SOD2), also known as MnSOD (manganese-dependent superoxide dismutase), is a 

mitochondrial enzyme that dismutates superoxide ions.

What is the molecular weight of SOD2? Is SOD2 post-translationally modified?

The molecular weight of SOD2 is 25 kDa. SOD2 acts as a homotetramer with manganese ion. SOD2 can be 

post-translationally modified, including phosphorylation, nitration, and acetylation. Phosphorylation of SOD2 

increases its enzymatic activity and occurs as part of the adaptive response to radiation stress (PMID: 23243068), 

while nitration and acetylation decrease SOD2 enzymatic activity (PMID: 20106845, 21172655).

What is the subcellular localization of superoxide dismutases?

SOD2 is the only superoxide dismutase that localizes to the mitochondrial matrix (PMID: 4578091). SOD1 is 

predominantly found in the cytoplasm and to a lesser extent in the mitochondrial intermembrane space and 

nucleus (PMID: 11507097), while SOD3 localizes extracellularly.

What is the role of SOD2 in neutralizing reactive oxygen species (ROS)?

Superoxide is a main form of ROS that originates directly from the electron transport chain in the mitochondrial 

matrix. SOD2 plays a crucial role in mitochondrial ROS homeostasis by catalyzing turnover of superoxide radicals 

to hydrogen peroxide and molecular oxygen. ROS are potent physiological signaling molecules but at high doses 

they contribute to the pathogenesis of many diseases, including cardiovascular and neurodegenerative diseases 

and cancer (PMID: 29669742). SOD2 also associates with mitochondrial DNA (mtDNA), protecting it from 

oxidative damage (PMID: 19228881).

What is the tissue expression pattern of SOD2?

SOD2 is ubiquitously expressed.

Can SOD2 be used as a marker of oxidative stress?

SOD2 is a key player in antioxidant defense and contributes to the regulation of oxidative stress. Defects in SOD2 

activity or levels can result in oxidative stress. However, SOD2 cannot be used as a marker of oxidative stress 

because its expression is not always altered by oxygen stress. 

Publikationen

Species	Application	Title
mouse	WB	Cell Metab; Exposure to Static Magnetic and Electric Fields Treats Type 2 Diabetes.; Authors - Calvin S Carter
mouse,human	WB	ACS Nano; Mesenchymal Stem Cell-Derived Extracellular Vesicles Attenuate Mitochondrial Damage and Inflammation by Stabilizing Mitochondrial DNA.; Authors - Meng Zhao
human	WB	Adv Sci (Weinh); IGFBP1 Sustains Cell Survival during Spatially-Confined Migration and Promotes Tumor Metastasis; Authors - Guoqing Cai
human	WB	Nat Commun; Suppression of mitochondrial ROS by prohibitin drives glioblastoma progression and therapeutic resistance.; Authors - Haohao Huang
mouse	WB	Redox Biol; miR-484 mediates oxidative stress-induced ovarian dysfunction and promotes granulosa cell apoptosis via SESN2 downregulation; Authors - Xiaofei Wang
mouse	WB	Nat Commun; The histone H3K9 methyltransferase SUV39H links SIRT1 repression to myocardial infarction.; Authors - Guang Yang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Macarena Lucia (Verified Customer) (10-17-2022)

clear band.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

Verwandte Produkte

SOD1 Polyclonal antibody

10269-1-AP

ubiquitin Polyclonal antibody

10201-2-AP

Catalase-Specific Polyclonal antibody

19792-1-AP

FOXO3A Monoclonal antibody

66428-1-Ig

SIRT1 Monoclonal antibody

60303-1-Ig

AKT Monoclonal antibody

60203-2-Ig