SOD2 Rekombinanter Antikörper

SOD2 Rekombinant Antikörper für IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240462F8

Kat-Nr. : 83519-2-RR

Synonyme

SODM, MNSOD, Mn SOD, IPOB, IPO B

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  IF Staining of HepG2 using 83519-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SOD2 antibody (83519-2-RR, Clone: 240462F8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HeLa using 83519-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SOD2 antibody (83519-2-RR, Clone: 240462F8 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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  FC experiment of HeLa using 83519-2-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug SOD2 Recombinant antibody (83519-2-RR, Clone:240462F8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83519-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83519-2-RR against Human SOD2 were performed. The affinity constant is 2.75 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83519-2-RR bindet in IF/ICC, FC (Intra), ELISA SOD2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SOD2 fusion protein Ag21388
• Vollständiger Name: superoxide dismutase 2, mitochondrial
• Berechnetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC016934
• Gene symbol: SOD2
• Gene ID (NCBI): 6648
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SOD2(superoxide dismutase 2, mitochondrial ) is also named as IPOB, MNSOD, SODM, Mn-SOD and belongs to the iron/manganese superoxide dismutase family. It is a marker of mitochondria, which is restricted to the perinuclear area in a cell with aggregate formation of mutant SOD1(PMID:12659845). It functions as the first line of antioxidant defense against highly reactive superoxide radicals and it appears to be early predictors for survival in septic patients with with MIF(PMID:20863520). It has 2 isoforms with the molecular weight of 25 kDa and 21 kDa. This antibody is specific to SOD2.