SOD3 Polyklonaler Antikörper

SOD3 Polyklonal Antikörper für IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 14316-1-AP

Synonyme

EC SOD, Extracellular superoxide dismutase, SOD3, Superoxide Dismutase 3

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Galerie der Validierungsdaten

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  WB analysis using 14316-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14316-1-AP (SOD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 14316-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14316-1-AP (SOD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 14316-1-AP

  IP Result of anti-SOD3 (IP:14316-1-AP, 4ug; Detection:14316-1-AP 1:1000) with human placenta tissue lysate 2800ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humane Urinprobe, humanes Herzgewebe, humanes Plazenta-Gewebe
• Erfolgreiche IP: humanes Plazenta-Gewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below

Produktinformation

14316-1-AP bindet in WB, IP, ELISA SOD3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SOD3 fusion protein Ag5556
• Vollständiger Name: superoxide dismutase 3, extracellular
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 25-33 kDa
• GenBank-Zugangsnummer: BC014418
• Gene symbol: SOD3
• Gene ID (NCBI): 6649
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SOD is located intracellular (SOD-1; cytosolic, SOD-2; mitochondrial matrix) and extracellular (SOD-3), and catalyzes the dismutation of superoxide anion to H2O2.SOD3, or EC-SOD, is the most recently characterized SOD, exists as a copper and zinc-containing tetramer, and is synthesized containing a signal peptide that directs this enzyme exclusively to extracellular spaces. SOD-3 is a Mn-containing tetrameric protein with a subunit Mr of 24-35 kDa. The activity of EC-SOD is dependent on its ability to form a tetramer of 135 kDa.

Publikationen

Species	Application	Title
mouse,human	WB	Br J Pharmacol; Inhibition of DNA methylation derepresses PPARγ and attenuates pulmonary fibrosis.; Authors - Ai Wei
human	WB	Transl Pediatr; Identification and evaluation of fructose-bisphosphate aldolase B as a potential diagnostic biomarker in choledochal cysts patients: a quantitative proteomic analysis.; Authors - Gedong Ming
human,rat	WB	Toxicol Appl Pharmacol; (-)-Epicatechin ameliorates cigarette smoke-induced lung inflammation via inhibiting ROS/NLRP3 inflammasome pathway in rats with COPD.; Authors - Xue Tian
human	WB	Clin Immunol; Oxidative stress is involved in immunosuppression and macrophage regulation in glioblastoma; Authors - Xisong Liang ; KD Validated